November 1, 2016

Tomáš Bárta Team

Research Interests | Publications | Funding | Team Members
tb
Mgr. Tomáš Bárta, PhD.

email: tbarta@med.muni.cz
phone: +420549496052
ResearchGate profile

Research interests

Our research is focused on small, non-coding RNA molecules called microRNA. Particularly, how they regulate cell fate decision and specification. MicroRNAs have emerged as critical regulatory components of the timing of stepwise events in development, typically by acting as modulators of molecular circuitries. Expression of miRNAs appears to be highly influenced by developmental stage and tissue specificity representing key regulatory elements of virtually all cell processes underlying cell function and identity. Although miRNAs represent crucial regulators of gene expression, their contribution to the regulation of development and cell fate decision is not fully understood.

Websites:
Stem Cell Biology Meeting – website for the conference that we organise once every two years.

miRNAsong – tool for generation and in silico testing of microRNA sponges.

DabLin – tool for lab management includes: database of cell lines, booking service for lab equipment, database of chemical reagents etc.

Department of Histology and Embryology – Web page of the Department of Histology and Embryology, Faculty of Medicine, Masaryk University (this website).

 


Publications

  • Melguizo-Sanchis D, Xu Y, Taheem D, Yu M, Tilgner K, Barta T, Gassner K, Anyfantis G, Wan T, Elango R, Alharthi S, El-Harouni AA, Przyborski S, Adam S, Saretzki G, Samarasinghe S, Armstrong L, Lako M. iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. Cell Death Dis. 2018 Jan 26;9(2):128. doi:10.1038/s41419-017-0141-1. PubMed PMID: 29374141; PubMed Central PMCID:PMC5833558.

  • Meader E, Barta T, Melguizo-Sanchis D, Tilgner K, Montaner D, El-Harouni AA, Armstrong L, Lako M. Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs. Stem Cells. 2018 Jan;36(1):55-64. doi: 10.1002/stem.2724. Epub 2017 Oct 27. PubMed PMID: 29047185; PubMed Central PMCID: PMC5765482.

  • Bagherpoor AJ, Dolezalova D, Barta T, Kučírek M, Sani SA, Ešner M, Kunova Bosakova M, Vinarský V, Peskova L, Hampl A, Štros M. Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins. Stem Cells Dev. 2017 Mar 1;26(5):328-340. doi: 10.1089/scd.2016.0274. Epub 2016 Dec 23. PubMed PMID: 27863459.

  • Barta T*, Peskova L, Hampl A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Sci Rep. 2016 Nov 18;6:36625. doi: 10.1038/srep36625. PubMed PMID: 27857164.


    * Corresponding author

  • Paige Taylor S, Kunova Bosakova M, Varecha M, Balek L, Barta T, Trantirek L, Jelinkova I, Duran I, Vesela I, Forlenza KN, Martin JH, Hampl A; University of Washington Center for Mendelian Genomics, Bamshad M, Nickerson D, Jaworski ML, Song J, Ko HW, Cohn DH, Krakow D, Krejci P. An inactivating mutation in intestinal cell kinase, ICK, impairs hedgehog signalling and causes short rib-polydactyly syndrome. Hum Mol Genet. 2016 Sep 15;25(18):3998-4011. doi: 10.1093/hmg/ddw240. Epub 2016 Jul 27. PubMed PMID: 27466187; PubMed Central PMCID: PMC5291234.

  • Barta T, Peskova L, Collin J, Montaner D, Neganova I, Armstrong L, Lako M. Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells. Stem Cells. 2016 Jan;34(1):246-51. doi: 10.1002/stem.2220. Epub 2015 Oct 9. PubMed PMID: 26418476; PubMed Central PMCID: PMC4982107.

  • Kollar P, Bárta T, Keltošová S, Trnová P, Müller Závalová V, Šmejkal K, Hošek J, Fedr R, Souček K, Hampl A. Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evid Based Complement Alternat Med. 2015;2015:251895. doi: 10.1155/2015/251895. Epub 2015 Feb 9. PubMed PMID: 25737734; PubMed Central PMCID: PMC4337268.

  • Jiang Y, Habibollah S, Tilgner K, Collin J, Barta T, Al-Aama JY, Tesarov L, Hussain R, Trafford AW, Kirkwood G, Sernagor E, Eleftheriou CG, Przyborski S, Stojković M, Lako M, Keavney B, Armstrong L. An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes. Stem Cells Transl Med. 2014 Apr;3(4):416-23. doi: 10.5966/sctm.2013-0105. Epub 2014 Mar 3. PubMed PMID: 24591732; PubMed Central PMCID: PMC3973710.

  • Augustyniak J, Zychowicz M, Podobinska M, Barta T, Buzanska L. Reprogramming of somatic cells: possible methods to derive safe, clinical-grade human induced pluripotent stem cells. Acta Neurobiol Exp (Wars). 2014;74(4):373-82. Review. PubMed PMID: 25576968.
  • Vinarsky V, Krivanek J, Rankel L, Nahacka Z, Barta T, Jaros J, Andera L, Hampl A. Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-induced apoptosis. Stem Cells Dev. 2013 Nov 15;22(22):2964-74. doi: 10.1089/scd.2013.0057. Epub 2013 Aug 2. PubMed PMID: 23806100; PubMed Central PMCID: PMC3822377.

  • Barta T, Dolezalova D, Holubcova Z, Hampl A. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture. Exp Biol Med (Maywood). 2013 Mar;238(3):271-5. doi: 10.1177/1535370213480711. Review. PubMed PMID: 23598972.

  • Kollar P, Bárta T, Hošek J, Souček K, Závalová VM, Artinian S, Talhouk R, Smejkal K, Suchý P Jr, Hampl A. Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response. Evid Based Complement Alternat Med. 2013;2013:350519. doi: 10.1155/2013/350519. Epub 2013 May 20. PubMed PMID: 23762124; PubMed Central PMCID: PMC3671669.

  • Krutá M, Bálek L, Hejnová R, Dobšáková Z, Eiselleová L, Matulka K, Bárta T, Fojtík P, Fajkus J, Hampl A, Dvořák P, Rotrekl V. Decrease in abundance of apurinic/apyrimidinic endonuclease causes failure of base excision repair in culture-adapted human embryonic stem cells. Stem Cells. 2013 Apr;31(4):693-702. doi: 10.1002/stem.1312. PubMed PMID: 23315699.

  • Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A. MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells. Stem Cells. 2012 Jul;30(7):1362-72. doi: 10.1002/stem.1108. PubMed PMID: 22511267.

  • Kollár P, Bárta T, Závalová V, Smejkal K, Hampl A. Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells. Br J Pharmacol. 2011 Apr;162(7):1534-41. doi: 10.1111/j.1476-5381.2010.01171.x. PubMed PMID: 21175584; PubMed Central PMCID: PMC3057291.

  • Holubcová Z, Matula P, Sedláčková M, Vinarský V, Doležalová D, Bárta T, Dvořák P, Hampl A. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011 Jan;29(1):46-56. doi: 10.1002/stem.549. PubMed PMID: 20960514.

  • Bárta T, Vinarský V, Holubcová Z, Dolezalová D, Verner J, Pospísilová S, Dvorák P, Hampl A. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010 Jul;28(7):1143-52. doi: 10.1002/stem.451. PubMed PMID: 20518019.


Funding

We are grateful for the support of our research:

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Team members

lp Lucie Pešková – Ph.D. Student
dj Denisa Jurčíková – B.Sc. Student

Tags

microRNA

mirRNA

sponges

microRNA sponges

mirna spoges

microRNA inhibitors

microRNA anti-sense

miRNAsong

cell reprogramming

miRNA sponge design

miRNA sponge testing