November 1, 2016

Tomáš Bárta Team

Research Interests | Publications | Funding | Team Members
tb
Mgr. Tomáš Bárta, PhD.

email: tbarta@med.muni.cz
phone: +420549496052
ResearchGate profile

Research interests

Our research is primarily focused on human pluripotent stem cells. Pluripotent stem cells have the unique capability to differentiate into the three primary germ cell layers and therefore potentially to produce any cell or tissue of the body. Like all stem cells, pluripotent stem cells are able to self-renew, representing a theoretically unlimited source of cells. Our group uses induced pluripotent stem cells derived from skin fibroblasts cells. We use commercially available fibroblasts and reprogram them into pluripotent state.

What do we study:
– Molecular mechanisms underlying cell fate switch during reprogramming with special focus on non-coding microRNA molecules.
– Molecular mechanisms of differentiation of human pluripotent stem cells into retinal tissues.
– How light irradiation regulates development of human retina
– How light irradiation regulates circadian rhythms/molecular clock pathways

Websites:
Stem Cell Biology Meeting – website for the conference that we organise once every two years.

miRNAsong – tool for generation and in silico testing of microRNA sponges.

DabLin – tool for lab management includes: database of cell lines, booking service for lab equipment, database of chemical reagents etc.

Department of Histology and Embryology – Web page of the Department of Histology and Embryology, Faculty of Medicine, Masaryk University (this website).

 


 

Publications

  • Volpato V, Smith J, Sandor C, Ried JS, Baud A, Handel A, Newey SE, Wessely F, Attar M, Whiteley E, Chintawar S, Verheyen A, Barta T, Lako M, Armstrong L, Muschet C, Artati A, Cusulin C, Christensen K, Patsch C, Sharma E, Nicod J, Brownjohn P, Stubbs V, Heywood WE, Gissen P, De Filippis R, Janssen K, Reinhardt P, Adamski J, Royaux I, Peeters PJ, Terstappen GC, Graf M, Livesey FJ, Akerman CJ, Mills K, Bowden R, Nicholson G, Webber C, Cader MZ, Lakics V. Reproducibility of Molecular Phenotypes after Long-Term Differentiation to Human iPSC-Derived Neurons: A Multi-Site Omics Study. Stem Cell Reports. 2018 Oct 9;11(4):897-911. doi: 10.1016/j.stemcr.2018.08.013. Epub 2018 Sep 20. PubMed PMID: 30245212; PubMed Central PMCID: PMC6178242.

  • Csukasi F, Duran I, Barad M, Barta T, Gudernova I, Trantirek L, Martin JH, Kuo CY, Woods J, Lee H, Cohn DH, Krejci P, Krakow D. The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling. Sci Transl Med. 2018 Sep 19;10(459). pii: eaat9356. doi: 10.1126/scitranslmed.aat9356. PubMed PMID: 30232230.

  • Renzova T, Bohaciakova D, Esner M, Pospisilova V, Barta T, Hampl A, Cajanek L. Inactivation of PLK4-STIL Module Prevents Self-Renewal and Triggers p53-Dependent Differentiation in Human Pluripotent Stem Cells. Stem Cell Reports. 2018 Oct 9;11(4):959-972. doi: 10.1016/j.stemcr.2018.08.008. Epub 2018 Sep 6. PubMed PMID: 30197118; PubMed Central PMCID: PMC6178195.

  • Porokh V, Vaňhara P, Bárta T, Jurečková L, Bohačiaková D, Pospíšilová V, Mináriková D, Holubcová Z, Pelková V, Souček K, Hampl A. Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells. Stem Cells Dev. 2018 Aug 15;27(16):1077-1084. doi: 10.1089/scd.2018.0017. Epub 2018 Jul 17. PubMed PMID: 29882484.

  • Melguizo-Sanchis D, Xu Y, Taheem D, Yu M, Tilgner K, Barta T, Gassner K, Anyfantis G, Wan T, Elango R, Alharthi S, El-Harouni AA, Przyborski S, Adam S, Saretzki G, Samarasinghe S, Armstrong L, Lako M. iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. Cell Death Dis. 2018 Jan 26;9(2):128. doi:10.1038/s41419-017-0141-1. PubMed PMID: 29374141; PubMed Central PMCID:PMC5833558.

  • Meader E, Barta T, Melguizo-Sanchis D, Tilgner K, Montaner D, El-Harouni AA, Armstrong L, Lako M. Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs. Stem Cells. 2018 Jan;36(1):55-64. doi: 10.1002/stem.2724. Epub 2017 Oct 27. PubMed PMID: 29047185; PubMed Central PMCID: PMC5765482.

  • Bagherpoor AJ, Dolezalova D, Barta T, Kučírek M, Sani SA, Ešner M, Kunova Bosakova M, Vinarský V, Peskova L, Hampl A, Štros M. Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins. Stem Cells Dev. 2017 Mar 1;26(5):328-340. doi: 10.1089/scd.2016.0274. Epub 2016 Dec 23. PubMed PMID: 27863459.

  • Barta T*, Peskova L, Hampl A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Sci Rep. 2016 Nov 18;6:36625. doi: 10.1038/srep36625. PubMed PMID: 27857164.


    * Corresponding author

  • Paige Taylor S, Kunova Bosakova M, Varecha M, Balek L, Barta T, Trantirek L, Jelinkova I, Duran I, Vesela I, Forlenza KN, Martin JH, Hampl A; University of Washington Center for Mendelian Genomics, Bamshad M, Nickerson D, Jaworski ML, Song J, Ko HW, Cohn DH, Krakow D, Krejci P. An inactivating mutation in intestinal cell kinase, ICK, impairs hedgehog signalling and causes short rib-polydactyly syndrome. Hum Mol Genet. 2016 Sep 15;25(18):3998-4011. doi: 10.1093/hmg/ddw240. Epub 2016 Jul 27. PubMed PMID: 27466187; PubMed Central PMCID: PMC5291234.

  • Barta T, Peskova L, Collin J, Montaner D, Neganova I, Armstrong L, Lako M. Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells. Stem Cells. 2016 Jan;34(1):246-51. doi: 10.1002/stem.2220. Epub 2015 Oct 9. PubMed PMID: 26418476; PubMed Central PMCID: PMC4982107.

  • Kollar P, Bárta T, Keltošová S, Trnová P, Müller Závalová V, Šmejkal K, Hošek J, Fedr R, Souček K, Hampl A. Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evid Based Complement Alternat Med. 2015;2015:251895. doi: 10.1155/2015/251895. Epub 2015 Feb 9. PubMed PMID: 25737734; PubMed Central PMCID: PMC4337268.

  • Jiang Y, Habibollah S, Tilgner K, Collin J, Barta T, Al-Aama JY, Tesarov L, Hussain R, Trafford AW, Kirkwood G, Sernagor E, Eleftheriou CG, Przyborski S, Stojković M, Lako M, Keavney B, Armstrong L. An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes. Stem Cells Transl Med. 2014 Apr;3(4):416-23. doi: 10.5966/sctm.2013-0105. Epub 2014 Mar 3. PubMed PMID: 24591732; PubMed Central PMCID: PMC3973710.

  • Augustyniak J, Zychowicz M, Podobinska M, Barta T, Buzanska L. Reprogramming of somatic cells: possible methods to derive safe, clinical-grade human induced pluripotent stem cells. Acta Neurobiol Exp (Wars). 2014;74(4):373-82. Review. PubMed PMID: 25576968.
  • Vinarsky V, Krivanek J, Rankel L, Nahacka Z, Barta T, Jaros J, Andera L, Hampl A. Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-induced apoptosis. Stem Cells Dev. 2013 Nov 15;22(22):2964-74. doi: 10.1089/scd.2013.0057. Epub 2013 Aug 2. PubMed PMID: 23806100; PubMed Central PMCID: PMC3822377.

  • Barta T, Dolezalova D, Holubcova Z, Hampl A. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture. Exp Biol Med (Maywood). 2013 Mar;238(3):271-5. doi: 10.1177/1535370213480711. Review. PubMed PMID: 23598972.

  • Kollar P, Bárta T, Hošek J, Souček K, Závalová VM, Artinian S, Talhouk R, Smejkal K, Suchý P Jr, Hampl A. Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response. Evid Based Complement Alternat Med. 2013;2013:350519. doi: 10.1155/2013/350519. Epub 2013 May 20. PubMed PMID: 23762124; PubMed Central PMCID: PMC3671669.

  • Krutá M, Bálek L, Hejnová R, Dobšáková Z, Eiselleová L, Matulka K, Bárta T, Fojtík P, Fajkus J, Hampl A, Dvořák P, Rotrekl V. Decrease in abundance of apurinic/apyrimidinic endonuclease causes failure of base excision repair in culture-adapted human embryonic stem cells. Stem Cells. 2013 Apr;31(4):693-702. doi: 10.1002/stem.1312. PubMed PMID: 23315699.

  • Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A. MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells. Stem Cells. 2012 Jul;30(7):1362-72. doi: 10.1002/stem.1108. PubMed PMID: 22511267.

  • Kollár P, Bárta T, Závalová V, Smejkal K, Hampl A. Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells. Br J Pharmacol. 2011 Apr;162(7):1534-41. doi: 10.1111/j.1476-5381.2010.01171.x. PubMed PMID: 21175584; PubMed Central PMCID: PMC3057291.

  • Holubcová Z, Matula P, Sedláčková M, Vinarský V, Doležalová D, Bárta T, Dvořák P, Hampl A. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011 Jan;29(1):46-56. doi: 10.1002/stem.549. PubMed PMID: 20960514.

  • Bárta T, Vinarský V, Holubcová Z, Dolezalová D, Verner J, Pospísilová S, Dvorák P, Hampl A. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010 Jul;28(7):1143-52. doi: 10.1002/stem.451. PubMed PMID: 20518019.

 


Funding

We are grateful for the support of our research:

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Team members

 

tereza Tereza Váňová – Postdoctoral Fellow
lucie Lucie Pešková – Ph.D. Student
dj Denisa Jurčíková – M.Sc. Student
livia Lívia Knapová – B.Sc. Student

Tags

microRNA

mirRNA

sponges

microRNA sponges

mirna spoges

microRNA inhibitors

microRNA anti-sense

miRNAsong

cell reprogramming

miRNA sponge design

miRNA sponge testing