November 1, 2016

Tomáš Bárta Team

Research Interests | Publications | Grants | Team Members
tb
Mgr. Tomáš Bárta, PhD.

email: tbarta@med.muni.cz
phone: +420549496052
ResearchGate profile

Research interests

MicroRNA sponges

MicroRNA (MiRNA) sponges are exogenously introduced transcripts containing multiple tandem high-affinity binding sites to a miRNA of interest. These miRNA-binding molecules are competitive regulators that sequester specific miRNAs, thus preventing miRNA/mRNA interaction. MiRNA sponges offer the advantage of inhibiting all seed family members and, when multiple MBSs are introduced, miRNA sponges can be used to inhibit a whole miRNA cluster. Given their advantages over other means of miRNA inhibition, miRNA sponges have the potential for use in disease treatment.
For better explanation of how microRNA sponges work, see the movie below and also our paper describing our tool for miRNA sponge construct design and testing.

 

 

 

 


Websites:

miRNAsong – tool for generation and in silico testing of microRNA sponges.

DabLin – tool for lab management includes: database of cell lines, booking service for lab equipment, database of chemical reagents etc.

Department of Histology and Embryology – Web page of the Department of Histology and Embryology, Faculty of Medicine, Masaryk University (this website).

 


Publications

  • Bagherpoor AJ, Dolezalova D, Barta T, Kucirek M, Sani SA, Esner M, Bosakova MK, Vinarsky V, Peskova L, Hampl A, Stros M. Properties of Human Embryonic Stem Cells and their Differentiated Derivatives Depend on Non-histone DNA-Binding HMGB1 and HMGB2 Proteins. Stem Cells Dev. 2016 Nov 18. [Epub ahead of print] PubMed PMID: 27863459.
  • Barta T*, Peskova L, Hampl A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Sci Rep. 2016 Nov 18;6:36625. doi: 10.1038/srep36625. PubMed PMID: 27857164.

    * Corresponding author

  • Paige Taylor S, Kunova Bosakova M, Varecha M, Balek L, Barta T, Trantirek L, Jelinkova I, Duran I, Vesela I, Forlenza KN, Martin JH, Hampl A; University Of Washington Center For Mendelian Genomics, Bamshad M, Nickerson D, Jaworski ML, Song J, Wan Ko H, Cohn DH, Krakow D, Krejci P. An inactivating mutation in intestinal cell kinase, ICK, impairs hedgehog signalling and causes short rib-polydactyly syndrome. Hum Mol Genet. 2016 Jul 27. pii: ddw240. [Epub ahead of print] PubMed PMID: 27466187.
  • Barta T, Peskova L, Collin J, Montaner D, Neganova I, Armstrong L, Lako M. Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells. Stem Cells. 2016 Jan;34(1):246-51. doi: 10.1002/stem.2220. Epub 2015 Oct 9. PubMed PMID: 26418476; PubMed Central PMCID: PMC4982107.

  • Kollar P, Bárta T, Keltošová S, Trnová P, Müller Závalová V, Šmejkal K, Hošek J, Fedr R, Souček K, Hampl A. Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evid Based Complement Alternat Med. 2015;2015:251895. doi: 10.1155/2015/251895. Epub 2015 Feb 9. PubMed PMID: 25737734; PubMed Central PMCID: PMC4337268.

  • Jiang Y, Habibollah S, Tilgner K, Collin J, Barta T, Al-Aama JY, Tesarov L, Hussain R, Trafford AW, Kirkwood G, Sernagor E, Eleftheriou CG, Przyborski S, Stojković M, Lako M, Keavney B, Armstrong L. An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes. Stem Cells Transl Med. 2014 Apr;3(4):416-23. doi: 10.5966/sctm.2013-0105. Epub 2014 Mar 3. PubMed PMID: 24591732; PubMed Central PMCID: PMC3973710.

  • Augustyniak J, Zychowicz M, Podobinska M, Barta T, Buzanska L. Reprogramming of somatic cells: possible methods to derive safe, clinical-grade human induced pluripotent stem cells. Acta Neurobiol Exp (Wars). 2014;74(4):373-82. Review. PubMed PMID: 25576968.

  • Vinarsky V, Krivanek J, Rankel L, Nahacka Z, Barta T, Jaros J, Andera L, Hampl A. Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-induced apoptosis. Stem Cells Dev. 2013 Nov 15;22(22):2964-74. doi: 10.1089/scd.2013.0057. Epub 2013 Aug 2. PubMed PMID: 23806100; PubMed Central PMCID: PMC3822377.

  • Barta T, Dolezalova D, Holubcova Z, Hampl A. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture. Exp Biol Med (Maywood). 2013 Mar;238(3):271-5. doi: 10.1177/1535370213480711. Review. PubMed PMID: 23598972.

  • Kollar P, Bárta T, Hošek J, Souček K, Závalová VM, Artinian S, Talhouk R, Smejkal K, Suchý P Jr, Hampl A. Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response. Evid Based Complement Alternat Med. 2013;2013:350519. doi: 10.1155/2013/350519. Epub 2013 May 20. PubMed PMID: 23762124; PubMed Central PMCID: PMC3671669.

  • Krutá M, Bálek L, Hejnová R, Dobšáková Z, Eiselleová L, Matulka K, Bárta T, Fojtík P, Fajkus J, Hampl A, Dvořák P, Rotrekl V. Decrease in abundance of apurinic/apyrimidinic endonuclease causes failure of base excision repair in culture-adapted human embryonic stem cells. Stem Cells. 2013 Apr;31(4):693-702. doi: 10.1002/stem.1312. PubMed PMID: 23315699.

  • Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A. MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells. Stem Cells. 2012 Jul;30(7):1362-72. doi: 10.1002/stem.1108. PubMed PMID: 22511267.

  • Kollár P, Bárta T, Závalová V, Smejkal K, Hampl A. Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells. Br J Pharmacol. 2011 Apr;162(7):1534-41. doi: 10.1111/j.1476-5381.2010.01171.x. PubMed PMID: 21175584; PubMed Central PMCID: PMC3057291.

  • Holubcová Z, Matula P, Sedláčková M, Vinarský V, Doležalová D, Bárta T, Dvořák P, Hampl A. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011 Jan;29(1):46-56. doi: 10.1002/stem.549. PubMed PMID: 20960514.

  • Bárta T, Vinarský V, Holubcová Z, Dolezalová D, Verner J, Pospísilová S, Dvorák P, Hampl A. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010 Jul;28(7):1143-52. doi: 10.1002/stem.451. PubMed PMID: 20518019.

 


Grants

Title: Indukce buněčné plasticity prostřednictvím modulace mikroRNA molekul: Nový přístup pro přeprogramování buněk (GJ16-24004Y)

MU Faculty/Unit: Masaryk University, Faculty of Medicine
Project Period: 1/2016 — 12/2018
Investor: Czech Science Foundation
Principal investigator: Tomáš Bárta

Title: The role of CDK1 in the regulation of the cell cycle in human embryonic stem cells (MUNI/E/0118/2009)

MU Faculty/Unit: Masaryk University, Faculty of Medicine
Project Period: 2009 — 2010
Investor: Masaryk University
Program framework: Rector’s programme to support MU student’s creative work.
Category E – development project + specific research
Principal investigator: Tomáš Bárta

Title: Analysis of molecular mechanisms underlying reaction of human embryonic stem cells to DNA damage. (MUNI/31/E0015/2007)

MU Faculty/Unit: Masaryk University, Faculty of Science
Project Period: 2007 — 2008
Investor: Masaryk University
Program framework: Rector’s programme to support MU student’s creative work.
Principal investigator: Tomáš Bárta

Team members

lp Lucie Pešková – Ph.D. Student
dj Denisa Jurčíková – B.Sc. Student

Tags

microRNA

mirRNA

sponges

microRNA sponges

mirna spoges

microRNA inhibitors

microRNA anti-sense

miRNAsong

cell reprogramming

miRNA sponge design

miRNA sponge testing