Comprehensive prognostic and predictive panel for chronic lymphocytic leukemia: a next-generation sequencing tool suitable for clinical practice and study of genetic architecture behind the disease progress

Information

This project doesn't include Faculty of Medicine. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

NV19-03-00091

Project Period

5/2019 - 12/2022

Investor / Pogramme / Project type

Ministry of Health of the CR

Ministry of Health Research Programme 2015 - 2022

MU Faculty or unit

Central European Institute of Technology

prof. RNDr. Šárka Pospíšilová, Ph.D.

Cooperating Organization

University Hospital Brno-Bohunice

Responsible person Mgr. Veronika Navrkalová, Ph.D.

Chronic lymphocytic leukemia (CLL) is common and still incurable leukemia of adults characterized by remarkable genetic and clinical heterogeneity. In proposed project we aim to study genetic background underlying clonal evolution of genomic alterations in treatment naïve CLL patients taking the advantage of availability of consecutive samples collected during disease course. Our main objective is to early disclose the disease progression and therapy need. We plan to assess both the prognostic and the predictive value of genomic aberrations detected just before treatment start. For this purpose, a highly complex next-generation sequencing (NGS) tool analyzing simultaneously both fundamental, as well as novel CLL biomarkers will be implemented. We employ benefits of capture-based NGS technology enabling to analyze in one test IGHV mutational status, recurrent chromosomal and gene defects, and Ig light chains clonality. We presume that our NGS panel tailored for CLL can be widely adopted in clinical practice as a simple comprehensive test for better patient prognostication.

Publications

Total number of publications: 44

2022

Integrated NGS testing and cfDNA analysis as an approach for aggregate tumor characterization and monitoring in a patient with aggressive lymphoma invading the central neural system.

NAVRKALOVÁ Veronika MAREČKOVÁ Andrea PORC Jakub Paweł JAROŠOVÁ Marie POSPÍŠILOVÁ Šárka KOTAŠKOVÁ Jana JANÍKOVÁ Andrea

Conference abstract

Year: 2022, type: Conference abstract
Komplexní analýza nádorového genomu pacientů s mnohočetným myelomem.

CHRÁST Lukáš FIDRICHOVÁ Anna BRYJOVÁ Lenka NAVRKALOVÁ Veronika POKRIVČÁKOVÁ Daniela MAREČKOVÁ Andrea BORSKÝ Marek ONDROUŠKOVÁ Eva OLTOVÁ Alexandra ŠMUHAŘOVÁ Petra POUR Luděk ŠTORK Martin KOTAŠKOVÁ Jana JAROŠOVÁ Marie

Conference abstract

Year: 2022, type: Conference abstract
Komplexní cytogenomická a molekulárně biologická analýza nádorového genomu pacientů s myeloidními malignitami se zaměřením na cílenou analýzu genu MECOM.

BRYJOVÁ Lenka ONDROUŠKOVÁ Eva ČÁBELOVÁ Kateřina ŠMEJKAL Jiří ŠMUHAŘOVÁ Petra KOTAŠKOVÁ Jana MAREČKOVÁ Andrea BOHÚNOVÁ Michaela JEŽÍŠKOVÁ Ivana FOLTA Adam SMITALOVÁ Dagmar DOUBEK Michael WEINBERGEROVÁ Barbora ČERVINEK Libor PANOVSKÁ Anna ŽÁČKOVÁ Daniela JAROŠOVÁ Marie

Conference abstract

Year: 2022, type: Conference abstract
LYNX: A web-based bioinformatic tool for targeted next-generation sequencing data analysis and visualization in lymphoid malignancies.

REIGL Tomáš PORC Jakub Paweł NAVRKALOVÁ Veronika HYNŠT Jakub PÁL Karol STRÁNSKÁ Kamila HRON T. HALBHUBER Z. DARZENTAS Nikos POSPÍŠILOVÁ Šárka PLEVOVÁ Karla

Conference abstract

Year: 2022, type: Conference abstract
Pathogenic germline variants of TP53 gene are rare in patients with chronic lymphocytic leukemia.

PAVLOVÁ Šárka MALČÍKOVÁ Jitka GOMBÍKOVÁ Johana ŠMARDOVÁ Jana PLEVOVÁ Karla KOTAŠKOVÁ Jana DVOŘÁČKOVÁ Barbara KUNT VONKOVÁ Barbara ZÁVACKÁ Kristýna NAVRKALOVÁ Veronika TRIZULJAK Jakub DOUBEK Michael POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Realita zapojení molekulárně biologických metod do diagnostiky a stratifikace lymfoproliferativních onemocnění.

KOTAŠKOVÁ Jana PLEVOVÁ Karla JAROŠOVÁ Marie POUR Luděk JANÍKOVÁ Andrea DOUBEK Michael POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Současná role cytogenomiky v diagnostice a prognostické stratifikaci hematologických malignit.

JAROŠOVÁ Marie KOTAŠKOVÁ Jana PLEVOVÁ Karla ONDROUŠKOVÁ Eva NAVRKALOVÁ Veronika BRYJOVÁ Lenka FIDRICHOVÁ Anna ČÁBELOVÁ Kateřina BOHÚNOVÁ Michaela ŠMEJKAL Jiří ŠMUHAŘOVÁ Petra OLTOVÁ Alexandra BORSKÝ Marek JEŽÍŠKOVÁ Ivana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Subclonal architectureof chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL

KOTAŠKOVÁ Jana KURUCOVÁ Terézia RÉBLOVÁ Kamila VALIŠOVÁ Martina ZÁVACKÁ Kristýna MAREČKOVÁ Andrea BOHÚNOVÁ Michaela PAVLOVÁ Šárka MALČÍKOVÁ Jitka NAVRKALOVÁ Veronika JAROŠOVÁ Marie DOUBEK Michael PLEVOVÁ Karla POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract

2021

A verified genomic reference sample for assessing performance of cancer panels detecting small variants of low allele frequency

JONES W. GONG B.S. NOVORADOVSKAYA N. LI D. KUSKO R. RICHMOND T.A. JOHANN D.J. BISGIN H. SAHRAEIAN S.M.E. BUSHEL P.R. PIROOZNIA M. WILKINS K. CHIERICI M. BAO W.J. BASEHORE L.S. LUCAS A.B. BURGESS D. BUTLER D.J. CAWLEY S. CHANG C.J. CHEN G.C. CHEN T. CHEN Y.C. CRAIG D.J. DEL POZO A. FOOX J. FRANCESCATTO M. FU YT FURLANELLO C. GIORDA K. GRIST K.P. GUAN M.J. HAO Y.Y. HAPPE S. HARIANI G. HASELEY N. JASPER J. JURMAN G. KREIL D.P. LABAJ P. LAI K. LI J.Y. LI Q.Z. LI Y.L. LI Z.G. LIU Z.C. LOPEZ M.S. MICLAUS K. MILLER R. MITTAL V.K. MOHIYUDDIN M. PABON-PENA C. PARSONS B.L. QIU F.J. SCHERER A. SHI T.L. STIEGELMEYER S. SUO C. TOM Nikola WANG D. WEN Z.N. WU L.H. XIAO W.Z. XU C. YU Y. ZHANG J.Y. ZHANG Y.F. ZHANG Z.H. ZHENG Y.T. MASON C.E. WILLEY J.C. TONG W.D. SHI L.M. XU J.

Article in Periodical

GENOME BIOLOGY, year: 2021, volume: 22, edition: 1, DOI
Bioinformatic strategies for the analysis of genomic aberrations detected by targeted NGS panels with clinical application

HYNŠT Jakub NAVRKALOVÁ Veronika PÁL Karol POSPÍŠILOVÁ Šárka

Article in Periodical

PeerJ, year: 2021, volume: 9, edition: MAR 2021, DOI