Project information
Comprehensive prognostic and predictive panel for chronic lymphocytic leukemia: a next-generation sequencing tool suitable for clinical practice and study of genetic architecture behind the disease progress

Information

This project doesn't include Faculty of Medicine. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

NV19-03-00091

Project Period

5/2019 - 12/2022

Investor / Pogramme / Project type

Ministry of Health of the CR

Ministry of Health Research Programme 2015 - 2022

MU Faculty or unit

Central European Institute of Technology

prof. RNDr. Šárka Pospíšilová, Ph.D.

Cooperating Organization

University Hospital Brno-Bohunice

Responsible person Mgr. Veronika Navrkalová, Ph.D.

Chronic lymphocytic leukemia (CLL) is common and still incurable leukemia of adults characterized by remarkable genetic and clinical heterogeneity. In proposed project we aim to study genetic background underlying clonal evolution of genomic alterations in treatment naïve CLL patients taking the advantage of availability of consecutive samples collected during disease course. Our main objective is to early disclose the disease progression and therapy need. We plan to assess both the prognostic and the predictive value of genomic aberrations detected just before treatment start. For this purpose, a highly complex next-generation sequencing (NGS) tool analyzing simultaneously both fundamental, as well as novel CLL biomarkers will be implemented. We employ benefits of capture-based NGS technology enabling to analyze in one test IGHV mutational status, recurrent chromosomal and gene defects, and Ig light chains clonality. We presume that our NGS panel tailored for CLL can be widely adopted in clinical practice as a simple comprehensive test for better patient prognostication.

Publications

Total number of publications: 46

2022

Subclonal architectureof chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL

KOTAŠKOVÁ Jana KURUCOVÁ Terézia RÉBLOVÁ Kamila VALIŠOVÁ Martina ZÁVACKÁ Kristýna MAREČKOVÁ Andrea BOHÚNOVÁ Michaela PAVLOVÁ Šárka MALČÍKOVÁ Jitka NAVRKALOVÁ Veronika JAROŠOVÁ Marie DOUBEK Michael PLEVOVÁ Karla POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract

2021

A verified genomic reference sample for assessing performance of cancer panels detecting small variants of low allele frequency

JONES W. GONG B.S. NOVORADOVSKAYA N. LI D. KUSKO R. RICHMOND T.A. JOHANN D.J. BISGIN H. SAHRAEIAN S.M.E. BUSHEL P.R. PIROOZNIA M. WILKINS K. CHIERICI M. BAO W.J. BASEHORE L.S. LUCAS A.B. BURGESS D. BUTLER D.J. CAWLEY S. CHANG C.J. CHEN G.C. CHEN T. CHEN Y.C. CRAIG D.J. DEL POZO A. FOOX J. FRANCESCATTO M. FU YT FURLANELLO C. GIORDA K. GRIST K.P. GUAN M.J. HAO Y.Y. HAPPE S. HARIANI G. HASELEY N. JASPER J. JURMAN G. KREIL D.P. LABAJ P. LAI K. LI J.Y. LI Q.Z. LI Y.L. LI Z.G. LIU Z.C. LOPEZ M.S. MICLAUS K. MILLER R. MITTAL V.K. MOHIYUDDIN M. PABON-PENA C. PARSONS B.L. QIU F.J. SCHERER A. SHI T.L. STIEGELMEYER S. SUO C. TOM Nikola WANG D. WEN Z.N. WU L.H. XIAO W.Z. XU C. YU Y. ZHANG J.Y. ZHANG Y.F. ZHANG Z.H. ZHENG Y.T. MASON C.E. WILLEY J.C. TONG W.D. SHI L.M. XU J.

Article in Periodical

GENOME BIOLOGY, year: 2021, volume: 22, edition: 1, DOI
Bioinformatic strategies for the analysis of genomic aberrations detected by targeted NGS panels with clinical application

HYNŠT Jakub NAVRKALOVÁ Veronika PÁL Karol POSPÍŠILOVÁ Šárka

Article in Periodical

PeerJ, year: 2021, volume: 9, edition: MAR 2021, DOI
Comparative analysis of targeted next-generation sequencing panels for the detection of gene mutations in chronic lymphocytic leukemia: an ERIC multi-center study

SUTTON L.A. LJUNGSTROM V. ENJUANES A. CORTESE D. SKAFTASON A. TAUSCH E. STAŇO KOZUBÍK Kateřina NADEU F. ARMAND M. MALČÍKOVÁ Jitka PANDZIC T. FORSTER J. DAVIS Z. OSCIER D. ROSSI D. GHIA P. STREFFORD J.C. POSPÍŠILOVÁ Šárka STILGENBAUER S. DAVI F. CAMPO E. STAMATOPOULOS K. ROSENQUIST R.

Article in Periodical

haematologica, year: 2021, volume: 106, edition: 3, DOI
Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions

GONG B.S. LI D. KUSKO R. NOVORADOVSKAYA N. ZHANG Y.F. WANG S.Z. PABON-PENA C. ZHANG Z.H. LAI K. CAI W.S. LOCOCO J.S. LADER E. RICHMOND T.A. MITTAL V.K. LIU L.C. JOHANN D.J. WILLEY J.C. BUSHEL P.R. YU Y. XU C. CHEN G.C. BURGESS D. CAWLEY S. GIORDA K. HASELEY N. QIU F.J. WILKINS K. ARIB H. ATTWOOLL C. BABSON K. BAO L.L. BAO W.J. LUCAS A.B. BEST H. BHANDARI A. BISGIN H. BLACKBURN J. BLOMQUIST T.M. BOARDMAN L. BURGHER B. BUTLER D.J. CHANG C.J. CHAUBEY A. CHEN T. CHIERICI M. CHIN C.R. CLOSE D. CONROY J. COLEMAN J.C. CRAIG D.J. CRAWFORD E. DEL POZO A. DEVESON I.W. DUNCAN D. ETEROVIC A.K. FAN X.H. FOOX J. FURLANELLO C. GHOSAL A. GLENN S. GUAN M.J. HAAG C. HANG X.Y. HAPPE S. HENNIGAN B. HIPP J. HONG H.X. HORVATH K. HU J.H. HUNG L.Y. JAROSZ M. KERKHOF J. KIPP B. KREIL D.P. LAPUNZINA P. LI P. LI Q.Z. LI W.H. LI Z.G. LIANG Y. LIU S.Q. LIU Z.C. MA C. MARELLA N. MARTIN-ARENAS R. MEGHERBI D.B. MENG Q.C. MIECZKOWSKI P.A. MORRISON T. MUZNY D. NING B.T. PARSONS B.L. PAWELETZ C.P. PIROOZNIA M. QU W.B. RAYMOND A. RINDLER P. RINGLER R. SADIKOVIC B. SCHERER A. SCHULZE E. SEBRA R. SHAKNOVICH R. SHI Q. SHI T.L. SILLA-CASTRO J.C. SMITH M. LOPEZ M.S. SONG P. STETSON D. STRAHL M. STUART A. SUPPLEE J. SZANKASI P. TAN H.W. TANG L.Y. TAO Y.H. THAKKAR S. THIERRY-MIEG D. THIERRY-MIEG J. THODIMA V.J. THOMAS D. TICHÝ Boris TOM Nikola GARCIA E.V. VERMA S. WALKER K. WANG C. WANG J.W. WANG Y.X. WEN Z.N. WIRTA V. WU L.H. XIAO C.L. XIAO W.Z. XU S.B. YANG M. YING J.M. YIP S.H. ZHANG G.L. ZHANG S. ZHAO M.R. ZHENG Y.T. ZHOU X.Y. MASON C.E. MERCER T. TONG W.D. SHI L.M. JONES W. XU J.S.

Article in Periodical

GENOME BIOLOGY, year: 2021, volume: 22, edition: 1, DOI
Dynamika mutací a chromozomálních aberací v závislosti na aktivitě CLL u neléčených pacientů.

NAVRKALOVÁ Veronika KOTAŠKOVÁ Jana VALIŠOVÁ Martina ŽENATOVÁ Marcela PANOVSKÁ Anna DOUBEK Michael POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2021, type: Conference abstract
Genomic landscape of B-other acute lymphoblastic leukemia in an adult retrospective cohort with a focus on BCR-ABL1-like subtype

HRABOVSKÝ Štěpán VRZALOVÁ Zuzana ŠTIKA Jiří JELINKOVA H. JAROŠOVÁ Marie NAVRKALOVÁ Veronika MARTENEK Jiří FOLBER František SALEK C. HORACEK JM. POSPÍŠILOVÁ Šárka MAYER Jiří DOUBEK Michael

Article in Periodical

Acta Oncologica, year: 2021, volume: 60, edition: 6, DOI
Higher-order connections between stereotyped subsets: implications for improved patient classification in CLL

AGATHANGELIDIS A. CHATZIDIMITRIOU A. GEMENETZI K. GIUDICELLI V. KARYPIDOU M. PLEVOVÁ Karla DAVIS Z. YAN X.J. JEROMIN S. SCHNEIDER C. PEDERSEN L.B. TSCHUMPER R.C. SUTTON L.A. BALIAKAS P. SCARFO L. VAN GASTEL E.J. ARMAND M. TAUSCH E. BIDERMAN B. BAER C. BAGNARA D. NAVARRO A. DE SEPTENVILLE A.L. GUIDO V. MITTERBAUER-HOHENDANNER G. DIMOVSKI A. BRIEGHEL C. LAWLESS S. MEGGENDORFER M. STRÁNSKÁ Kamila RITGEN M. FACCO M. TRESOLDI C. VISENTIN A. PATRIARCA A. CATHERWOOD M. BONELLO L. SUDARIKOV A. VANURA K. ROUMELIOTI M. FRANCOVA H.S. MOYSIADIS T. VERONESE S. GIANNOPOULOS K. MANSOURI L. KARAN-DJURASEVIC T. SANDALTZOPOULOS R. BODOR C. FAIS F. KATER A. PANOVSKA I. ROSSI D. ALSHEMMARI S. PANAGIOTIDIS P. COSTEAS P. ESPINET B. ANTIC D. FORONI L. MONTILLO M. TRENTIN L. STAVROYIANNI N. GAIDANO G. DI CELLE P.F. NIEMANN C. CAMPO E. ANAGNOSTOPOULOS A. POTT C. FISCHER K. HALLEK M. OSCIER D. STILGENBAUER S. HAFERLACH C. JELINEK D. CHIORAZZI N. POSPÍŠILOVÁ Šárka LEFRANC M.P. KOSSIDA S. LANGERAK A.W. BELESSI Cx. DAVI F. ROSENQUIST R. GHIA P. STAMATOPOULOS K.

Article in Periodical

Blood, year: 2021, volume: 137, edition: 10, DOI
Klinický dopad a evoluce minoritních TP53-mutovaných klonů v kontextu různých terapeutických režimů u CLL.

MALČÍKOVÁ Jitka PAVLOVÁ Šárka KUNT VONKOVÁ Barbara RADOVÁ Lenka PLEVOVÁ Karla KOTAŠKOVÁ Jana PÁL Karol DVOŘÁČKOVÁ Barbara ŽENATOVÁ Marcela HYNŠT Jakub ONDROUŠKOVÁ Eva PANOVSKÁ Anna MAŠLEJOVÁ Stanislava BEZDĚKOVÁ Lucie ZUCHNICKÁ J. URBANOVÁ R. LYSÁK Daniel NOVÁK J. BREJCHA Martin DOUBEK Michael

Conference abstract

Year: 2021, type: Conference abstract
Low-burden TP53 mutations in CLL: clinical impact and clonal evolution within the context of different treatment options

MALČÍKOVÁ Jitka PAVLOVÁ Šárka KUNT VONKOVÁ Barbara RADOVÁ Lenka PLEVOVÁ Karla KOTAŠKOVÁ Jana PÁL Karol DVOŘÁČKOVÁ Barbara ŽENATOVÁ Marcela HYNŠT Jakub ONDROUŠKOVÁ Eva PANOVSKÁ Anna BRYCHTOVÁ Yvona ZÁVACKÁ Kristýna TICHÝ Boris TOM Nikola MAYER Jiří DOUBEK Michael POSPÍŠILOVÁ Šárka

Article in Periodical

Blood, year: 2021, volume: 138, edition: 25, DOI