Laser-induced fluorescence spectroscopy in tissue local necrosis detection

Authors

CIP Ondrej BUCHTA Zdenek LESUNDAK Adam RANDULA Antonin MIKEL Bretislav LAZAR Josef VEVERKOVÁ Lenka

Year of publication 2014
Type Article in Proceedings
Conference OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1117/12.2052637
Field Optics, masers and lasers
Keywords IndoCyanine Green; fluorescence spectroscopy; small intestine; surgery
Description The recent effort leads to reliable imaging techniques which can help to a surgeon during operations. The fluorescence spectroscopy was selected as very useful online in vivo imaging method to organics and biological materials analysis. The presented work scopes to a laser induced fluorescence spectroscopy technique to detect tissue local necrosis in small intestine surgery. In first experiments, we tested tissue auto-fluorescence technique but a signal-to-noise ratio didn't express significant results. Then we applied a contrast dye - IndoCyanine Green (ICG) which absorbs and emits wavelengths in the near IR. We arranged the pilot experimental setup based on highly coherent extended cavity diode laser (ECDL) used for stimulating of some critical areas of the small intestine tissue with injected ICG dye. We demonstrated the distribution of the ICG exciter with the first file of shots of small intestine tissue of a rabbit that was captured by high sensitivity fluorescent cam
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