Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum

Authors

PLESKAČOVÁ Anna TOMANDL Josef BREJCHA Stanislav PÁCAL Lukáš KAŇKOVÁ Kateřina

Year of publication 2016
Type Conference abstract
Citation
Description Uric acid (UA) is a terminal metabolite of purine metabolism generated from xanthine (X) and hypoxanthine (HX) by the action of xanthine oxidoreductase (XOR) as a rate limiting enzyme. An increase of XOR activity has been reported under several pathophysiological conditions [1] and elevated UA and X levels are important risk factors of several diseases including those related to lifestyle [2]. On the other hand UA is considered as an important antioxidant in vivo [3]. UA is usually determined in serum using an enzyme uricase in clinical chemistry laboratory. There are several disadvantages of this commonly used enzyme method, firstly it does not provide data on X and HX levels reflecting the activity of XOR, secondly hyperxanthinemia may result in false positive results and thirdly uricase requires bivalent ions and thus this method is not appropriate for EDTA-plasma samples. The aim of this study was to optimize an HPLC determination of UA, X and HX convenient for both human EDTA-plasma and serum.
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