The chromosome-level genome sequence and karyotypic evolution of Megadenia pygmaea (Brassicaceae)

Warning

This publication doesn't include Faculty of Medicine. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors

YANG W.J. ZHANG L. MANDÁKOVÁ Terezie HUANG L. LI T. JIANG J.B. YANG Y.Z. LYSÁK Martin LIU J.Q. HU Q.J.

Year of publication 2021
Type Article in Periodical
Magazine / Source Molecular Ecology Resources
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13291
Doi http://dx.doi.org/10.1111/1755-0998.13291
Keywords Brassicaceae; descending dysploidy; genome assembly; karyotype and chromosome evolution; Qinghai-Tibet Plateau
Description Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was assembled for Megadenia pygmaea using Nanopore long reads and high-throughput chromosome conformation capture (Hi-C). The assembled genome is 215.2 Mb and is anchored on six pseudochromosomes. We annotated a total of 25,607 high-confidence protein-coding genes and corroborated the phylogenetic affinity of Megadenia with the Brassicaceae expanded lineage II, containing numerous agricultural crops. We dated the divergence of Megadenia from its closest relatives to 27.04 (19.11-36.60) million years ago. A reconstruction of the chromosomal composition of the species was performed based on the de novo assembled genome and comparative chromosome painting analysis. The karyotype structure of M. pygmaea is very similar to the previously inferred proto-Calepineae karyotype (PCK; n = 7) of the lineage II. However, an end-to-end translocation between two ancestral chromosomes reduced the chromosome number from n = 7 to n = 6 in Megadenia. Our reference genome provides fundamental information for karyotypic evolution and evolutionary study of this genus.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info