A simple dilute-and-shoot procedure for the determination of platinum in human pleural effusions using HR-CS GF-AAS

Authors

ZVĚŘINA Ondřej VENCLÍČEK Ondřej KUTA Jan COUFALÍK Pavel HAGAROVÁ Ingrid BRAT Kristián

Year of publication 2021
Type Article in Periodical
Magazine / Source Journal of Trace Elements in Medicine and Biology
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.sciencedirect.com/science/article/pii/S0946672X21001590?dgcid=author
Doi http://dx.doi.org/10.1016/j.jtemb.2021.126869
Keywords Lung cancer; Platinum; Pleural effusion; HR-CS GF-AAS
Description Background High-resolution continuum source AAS is an emerging technique for the determination of trace elements in clinical analysis. We aimed to develop a method for the direct determination of platinum (Pt) in pleural effusions that could deepen the understanding of the dynamics of intrapleural Pt concentration during cytostatic therapy. Materials and methods Samples were collected by thoracic drainage from five patients with lung cancer undergoing platinum based chemotherapy. A simple dilute-and-shoot method for Pt determination in the pleural effusions was developed. Ashing of the sample in an oxygen flow in a graphite tube allowed for direct analysis without prior mineralization. The trueness of the method was verified using an independent technique (ICP-MS). As platinum derivatives are only active in its free form (not bound to proteins), Pt in samples was further divided into free and protein-bound forms by means of ultrafiltration. Results Using the proposed method, Pt contents (free and total) were determined in samples collected at different times after the intravenous application of the Pt derivative. The concentration of total Pt reached values of up to 5,000 µg/L and different patterns of its dynamics in intrapleural fluid were observed. Conclusions The developed method enables the fast and simple determination of Pt in biological fluids. It may be applied on a large scale to improve the understanding of Pt dynamics during cytostatic therapy, and also to determine the optimal timing of both thoracic drainage and administration of systemic chemotherapy.
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