Derivation and characterization of clinical-grade human embryonic stem cells

Authors

SOURALOVÁ Tereza HOLUBCOVÁ Zuzana KYJOVSKÁ Drahomíra HAMPL Aleš KOUTNÁ Irena

Year of publication 2021
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description Human embryonic stem cells (hESCs) represent an unlimited source of cells for the differentiation into the derivates that are suitable for a variety of biomedical applications. Cell therapies, disease modeling, and drug testing, which are examples of application, demand robust and replicable protocols for the derivation and differentiation of hESCs. Nevertheless, allogeneic background and undefined culture conditions are limitations of current protocols. Therefore, here we present the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos (excess for fertility treatment) under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinicalgrade hESC lines for a variety of clinical applications.
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