Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Authors

TURČANOVÁ Michaela HRTOŇ Martin DVOŘÁK Petr NOVÁK Kamil HERMANOVÁ Markéta BEDNAŘÍK Zdeněk POLZER Stanislav BURŠA Jiří

Year of publication 2021
Type Article in Periodical
Magazine / Source BioMed Research International
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.hindawi.com/journals/bmri/2021/6879765/
Doi http://dx.doi.org/10.1155/2021/6879765
Description A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info