Role of HPV and protein p16 in patients with OSCC – three years clinical experience
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| Year of publication | 2013 |
| Type | Conference abstract |
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| Description | The most common tumor of head and neck is oral squamous cell carcinoma (OSCC). OSCC occurs in the Czech Republic with the incidence of 4.2 cases per 100 000 patients-year and mortality of 2.2 cases per 100 000 patients-year. The majority of patients unfortunately comes with advanced finding. The incidence and the mortality of OSCC are practically same during the last 20 years. Recently we have noticed the changing trend in the age structure of patients that is associated with the change of risk factors. The head and neck carcinoma is connected with a Human papillomavirus (HPV). We know over 130 HPV types – these are classified as low-risk or high-risk. High-risk types can lead to cancers of oropharynx or oral cavity. Many surveys proved that the beginning of oropharyngeal cancers (OPCS) is etiologically associated with this high-risk HPV, but in OSCC was presence of HPV uncommon. Most studies have shown high prevalence of HPV in OPCS (73%), but on the other hand patients with OSCC have low infection rate of HPV (4%). Fifty two cases of OSCC treated at our department during period 2011-2013 were analysed for presence of HPV. The HPV were confirmed only in three samples, two patients were positive for low-risk HPV, one patient for high-risk HPV. HPV status was determined by conventional polymerase chain reaction (PCR) from native samples. We detected the overexpression of protein p16 in 7 samples, but not in samples, which were HPV positive. P16 was detected from formalin-fixed, paraffin-embedded tissues by immunohistochemical staining. Our clinical experience shows different prevalence of HPV positivity between OPCS and OSCC. |