Inflammatory profiling of the satellite glial cells in the dorsal root ganglia and their contribution to induction and maintenance of neuropathic pain
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| Year of publication | 2014 |
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| Description | Peripheral neuropathic pain (PNP), manifested as spontaneous pain and hyperalgesia, arises as a result of many forms of peripheral nerve damage. There is compelling evidence indicating that hyperalgesia and ongoing pain due to peripheral nerve injury are associated with cellular and molecular changes of the dorsal root ganglia (DRG) including activation of satellite glial cells (SGC) that control excitability of the primary sensory neurons. Most frequently used experimental models of PNP are based on chronic constriction injury (CCI) or spared nerve injury (SNI) of rat sciatic nerve. Recently, an immunohistochemical detection of protein expression is the only methodological approach for cellular imaging of in situ proteins distribution and differentiation of their regulation between the neurons and SGC. Therefore, we used immunohistochemical detection of Toll-like receptors, proinflammatory cytokines (IL-1b, TNFa, IL-6), their receptors and regulatory proteins for investigation of induction and regulation of inflammatory profiling of SGC in DRG of PNP models. Our results indicate that inflammatory reactions of SGC differ from DRG neurons and may significantly contribute to their hyperexcitation as well as to conditioning of non-impaired neurons surviving primary injury to next impairment. |
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