Retinoic acid analysis by mass spectrometry

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Authors	HAJTMANOVÁ Kristína KUČERA Lukáš KOLÁŘOVÁ Lenka PEŇA MENDÉZ Eladia Maria VAŇHARA Petr HAVEL Josef
Year of publication	2015
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	Retinoic acid (RA) is a vitamin A (retinol)-derived, small lipophilic molecule, involved in cell proliferation, differentiation or histogenesis, with important clinical applications, e.g. in leukemia treatment. In this work, we have analyzed retinoic acid by Laser Desorption Ionization (LDI), Surface Assisted Laser Desorption Ionization (SALDI) or Matrix Assisted Laser Desorption Time Of Flight Mass spectrometry (MALDI TOF MS). A formation of cation radical RA•+ using LDI or the protonated form RA.H+ in MALDI was observed. In addition, we developed and optimized SALDI of RA using various forms of gold nano-particles.[1] Applying SALDI or combination of nano-particles and common matrices (SALDI-MALDI) allowed a highly sensitive determination of RA. In summary, analysis and determination of RA by MS was optimized, allowing for subsequent studies in cells or tissues.