Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment

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This publication doesn't include Faculty of Medicine. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors

FARKA Zdeněk JUŘÍK Tomáš PASTUCHA Matěj KOVÁŘ David LACINA Karel SKLÁDAL Petr

Year of publication 2016
Type Article in Periodical
Magazine / Source Electroanalysis
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full
Doi http://dx.doi.org/10.1002/elan.201600093
Field Biochemistry
Keywords Atomic force microscopy; Biosensor; Immunosensor; Label-free detection; Salmonella Typhimurium
Description A label-free immunosensor for rapid detection of Salmonella Typhimurium based on electrochemical impedance spectroscopy was developed. Specific antibody was immobilized to a screen-printed electrode via cysteamine monolayer activated with glutaraldehyde and the impedance was measured between two gold electrodes. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. Wide linear response was obtained in the range between 10^3 CFU·mL-1 and 10^8 CFU·mL-1. The successful detection of Salmonella in spiked milk demonstrates the suitability of sensor for the analysis of real samples.
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