Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL

Investor logo

Warning

This publication doesn't include Faculty of Medicine. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors

MRÁZKOVÁ Jana MALINOVSKÁ Lenka WIMMEROVÁ Michaela

Year of publication 2017
Type Chapter of a book
MU Faculty or unit

Faculty of Science

Citation
Description In this chapter, we describe a site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa . Moreover, procedures for the production and purification of PA-IIL mutants are described, and several basic methods for characterizing the mutants are discussed.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info