Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

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Authors	MANDÁKOVÁ Terezie LYSÁK Martin
Year of publication	2016
Type	Article in Periodical
Magazine / Source	Current protocols in plant biology
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
Doi	http://dx.doi.org/10.1002/cppb.20022
Field	Genetics and molecular biology
Keywords	BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes
Description	Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.
Related projects:	CEITEC 2020