Restrictions in the T-cell repertoire of chronic lymphocytic leukemia: high-throughput immunoprofiling supports selection by shared antigenic elements

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Authors	VARDI A. VLACHONIKOLA E. KARYPIDOU M. STALIKA E. BIKOS Vasileios GEMENETZI K. MARAMIS C. SIORENTA A. ANAGNOSTOPOULOS A. POSPÍŠILOVÁ Šárka MAGLAVERAS N. CHOUVARDA I. STAMATOPOULOS K. HADZIDIMITRIOU A.
Year of publication	2017
Type	Article in Periodical
Magazine / Source	Leukemia
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://www.nature.com/leu/journal/v31/n7/full/leu2016362a.html
Doi	http://dx.doi.org/10.1038/leu.2016.362
Field	Oncology and hematology
Keywords	CLONAL EVOLUTION; CONVERGENT RECOMBINATION; TARGETED THERAPIES; MOUSE MODEL; RECEPTORS; LENALIDOMIDE; IBRUTINIB; DRIVEN; CLL; CHEMOIMMUNOTHERAPY
Attached files	vardi2016.pdf
Description	Immunoglobulin (IG) gene repertoire restrictions strongly support antigen selection in the pathogenesis of chronic lymphocytic leukemia (CLL). Given the emerging multifarious interactions between CLL and bystander T cells, we sought to determine whether antigen(s) are also selecting T cells in CLL. We performed a large-scale, next-generation sequencing (NGS) study of the T-cell repertoire, focusing on major stereotyped subsets representing CLL subgroups with undisputed antigenic drive, but also included patients carrying non-subset IG rearrangements to seek for T-cell immunogenetic signatures ubiquitous in CLL. Considering the inherent limitations of NGS, we deployed bioinformatics algorithms for qualitative curation of T-cell receptor rearrangements, and included multiple types of controls. Overall, we document the clonal architecture of the T-cell repertoire in CLL. These T-cell clones persist and further expand overtime, and can be shared by different patients, most especially patients belonging to the same stereotyped subset. Notably, these shared clonotypes appear to be disease-specific, as they are found in neither public databases nor healthy controls. Altogether, these findings indicate that antigen drive likely underlies T-cell expansions in CLL and may be acting in a CLL subset-specific context. Whether these are the same antigens interacting with the malignant clone or tumor-derived antigens remains to be elucidated.
Related projects:	CEITEC - open access CEITEC 2020