Visualization of stable ferritin complexes with palladium, rhodium and iridium nanoparticles detected by their catalytic activity in native polyacrylamide gels

Authors

PEKAŘÍK Vladimír PEŠKOVÁ Marie GURAN R. NOVÁČEK Jiří HEGER Z. TRIPSIANES Konstantinos KUMAR Jitender ADAM V.

Year of publication 2017
Type Article in Periodical
Magazine / Source Dalton Transactions
MU Faculty or unit

Faculty of Medicine

Citation
Web http://dx.doi.org/10.1039/C7DT02818K
Doi http://dx.doi.org/10.1039/C7DT02818K
Field Biochemistry
Keywords stable ferritin complexes
Attached files
Description The reductive discoloration of azo dye, Congo red, catalyzed by noble metal nanoparticles was used to visualize protein–metal complexes in native polyacrylamide gels after counterstaining with Coomassie blue. This technique was used to characterize the synthesis of palladium, rhodium and iridium nanoparticles encapsulated in Pyrococcus furiosus ferritin.
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