Rapid identification of staphylococci by Raman spectroscopy

Authors

REBROŠOVÁ Katarína ŠILER M. SAMEK O. RŮŽIČKA Filip BERNATOVÁ S. HOLÁ Veronika JEŽEK J. ZEMÁNEK P. SOKOLOVÁ J. PETRÁŠ P.

Year of publication 2017
Type Article in Periodical
Magazine / Source Scientific Reports
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1038/s41598-017-13940-w
Field Microbiology, virology
Keywords bacterium colony; human major clinical study; nonhuman ; Raman spectrometry; Staphylococcus
Description Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
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