Hymenobacter amundsenii sp. nov. resistant to ultraviolet radiation, isolated from regoliths in Antarctica

Warning

This publication doesn't include Faculty of Medicine. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors

SEDLÁČEK Ivo PANTŮČEK Roman KRÁLOVÁ Stanislava MAŠLAŇOVÁ Ivana HOLOCHOVÁ Pavla STAŇKOVÁ Eva VRBOVSKÁ Veronika ŠVEC Pavel BUSSE Hans Jürgen

Year of publication 2019
Type Article in Periodical
Magazine / Source Systematic and Applied Microbiology
MU Faculty or unit

Faculty of Science

Citation
Web Full Text
Doi http://dx.doi.org/10.1016/j.syapm.2018.12.004
Keywords Hymenobacter amundsenii; Antarctica; Soil bacteria; Regolith; UV-radiation resistance; Species description
Attached files
Description A group of thirteen bacterial strains was isolated from rock samples collected in a deglaciated northern part of James Ross Island, Antarctica. The cells were rod-shaped, Gram-stain-negative, non-motile, catalase positive, and produced moderately slimy, ultraviolet light (UVC)-irradiation-resistant and red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, extensive biotyping, fatty acid profile, chemotaxonomy analyses, and whole genome sequencing were applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all isolates constituted a coherent group belonging to the genus Hymenobacter. The closest relatives to the representative isolate P5136(T) were Hymenobacter psychrophilus BZ33r(T) and Hymenobacter rubripertinctus CCM 8852(T), exhibiting 97.53% and 97.47% 16S rRNA pairwise similarity, respectively. Average nucleotide identity calculated from the whole-genome sequencing data supported the finding that P5136(T) represents a distinct Hymenobacter species. The major components in fatty acid profiles were Summed Feature 3 (C-16:1 omega 7c/C-16:1 omega 6c), C-16:1 omega 5c, C-15:0 iso and C-15:0 anteiso. The cellular quinone content contained unanimously menaquinone MK-6 and MK-7 (ratio 1:5.1). The predominant polar lipid was phosphatidylethanolamine, and moderate to minor amounts of two unknown polar lipids, two unknown aminolipids, one unknown glycolipid and two unknown glycophospholipids were present. The G + C content of genomic DNAs is 60.31 mol%. Based on all the obtained results, we propose a novel species for which the name Hymenobacter amundsenii sp. nov. is suggested, with the type strain P5136(T) (= CCM 8682(T) =LMG 29687(T)). (C) 2018 Elsevier GmbH. All rights reserved.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info