High level of circulat ing microparticles in patients with BCR/ ABL negative myeloproliferative neoplasm - A pilot study

Title in English Vysoká hladina cirkulujících mikropartikulí u pacientu s BCR/ ABL negativními myeloproliferativními chorobami - Pilotní studie

ASWAD Mohamed Hussam KISSOVÁ Jarmila ŘÍHOVÁ Lucie ZAVŘELOVÁ Jiřina OVESNÁ Petra PENKA Miroslav

Year of publication 2019
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Faculty of Medicine

Web http://dx.doi.org/10.14735/amko2019109
Doi http://dx.doi.org/10.14735/amko2019109
Keywords Janus Kinase 2; microparticles; myeloproliferative disorders; procoagulation activity; thrombosis
Description BACKGROUND: Microparticles (MPs) are small (0.1-1 [MU]m) cell-derived vesicles released during activation or apoptosis, with a surface-exposed phosphatidylserine along with antigens indicating the cellular origin. The level of MPs is known to be elevated in thromboembolic diseases and malignancies; it is believed that MPs are not only amplifying but can also initiate the thrombogenesis processes. BCR/ABL negative myeloproliferative neoplasms (MPNs) are clonal haematopoietic diseases, which include polycythemia vera, essential thrombocythemia and primary myelofibrosis. One of the main problems of MPN patients is high risk and incidence of thrombosis which affect the survival, quality of life and life expectancy. PATIENTS AND METHODS: The clinical significance of circulating MPs was assessed in a group of 179 patients with BCR/ABL-negative MPNs. Analysis of MPs was done using flow cytometry on 417 samples, and MPs procoagulation activity was performed using a functional assay called Zymuphen MP-activity (Hyphen Biomed, Neuville-sur-oise, France) on 274 samples. RESULTS: Significantly higher absolute and relative count of platelet MPs was found in MPN patients when compared with healthy group, respectively (p = 0.001, p = 0.043). Erythrocyte MPs were also significantly higher in MPN patients than in the healthy group (p < 0.001). Procoagulation activity of MPs was as well significantly higher in patients compared to the control group (p < 0.001). Patients with primary myelofibrosis had decreased absolute and relative count of platelet MPs compared to polycythemia vera and essential thrombocythemia patients, respectively (p = 0.008, p = 0.014). Presence of JAK2V617F mutation was associated with higher absolute and relative count of platelet MPs, respectively (p = 0.045, p = 0.029). CONCLUSION: Although some literature data support the hypothesis of a direct relation between MPs and thrombotic events in MPN patients, further studies are needed to evaluate the clinical implication of MPs in the hypercoagulation state of MPN patients.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info