Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells

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Authors

KRATOCHVÍLOVÁ Adéla VESELÁ Barbora LEDVINA Vojtěch ŠVANDOVÁ Eva KLEPÁRNÍK Karel DADÁKOVÁ Kateřina BENEŠ Petr MATALOVÁ Eva

Year of publication 2020
Type Article in Periodical
Magazine / Source Scientific Reports
MU Faculty or unit

Faculty of Science

Citation
Web https://www.nature.com/articles/s41598-020-64294-9
Doi http://dx.doi.org/10.1038/s41598-020-64294-9
Keywords Bone; Bone development; Cell biology; Proteases
Description Caspases are proteases traditionally associated with inflammation and cell death. Recently, they have also been shown to modulate cell proliferation and differentiation. The aim of the current research was to search for osteogenic molecules affected by caspase inhibition and to specify the individual caspases critical for these effects with a focus on proapoptotic caspases: caspase-2, -3, -6, -7, -8 and -9. Along with osteocalcin (Ocn), general caspase inhibition significantly decreased the expression of the Phex gene in differentiated MC3T3-E1 cells. The inhibition of individual caspases indicated that caspase-8 is a major contributor to the modification of Ocn and Phex expression. Caspase-2 and-6 had effects on Ocn and caspase-6 had an effect on Phex. These data confirm and expand the current knowledge about the nonapoptotic roles of caspases and the effect of their pharmacological inhibition on the osteogenic potential of osteoblastic cells.
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