miR-181a-2*expression is different amongst carcinomas from the colorectal serrated route

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Authors

KONDELOVÁ Alexandra ALBURQUERQUE-GONZALEZ B. VYCHYTILOVÁ Petra GARCIA-SOLANO J. PROCHAZKA V. KALA Z. PEREZ F. SLABÝ Ondřej CONESA-ZAMORA P.

Year of publication 2020
Type Article in Periodical
Magazine / Source MUTAGENESIS
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://academic.oup.com/mutage/article-abstract/35/3/233/5648152?redirectedFrom=fulltext
Doi http://dx.doi.org/10.1093/mutage/gez039
Keywords CPG ISLAND METHYLATION; MICROSATELLITE INSTABILITY; BRAF MUTATION; CANCER; EXPRESSION; POLYPS; ADENOCARCINOMA; MICRORNA-31; BIOMARKERS; ADENOMAS
Description Serrated adenocarcinoma (SAC) and colorectal carcinomas showing histological and molecular features of high-level of microsatellite instability (hmMSI-H) are both end points of the serrated pathway of colorectal carcinogenesis. Despite common features (right-sided location, CpG island methylation phenotype and BRAF mutation) there are no studies comparing the microRNA (miRNA) expression profiles in SACs and hmMSI-H.The microtranscriptome from 12 SACs and 8 hmMSI-H were analysed using Affymetrix GeneChip miRNA 3.0 arrays and differentially enriched functions involving immune response were observed from this comparison. miR-181a-2* was found significantly more expressed in hmMSI-H than in SAC and higher expression of this miRNA in microsatellite unstable colorectal cancer were corroborated by Real-Time PCR in an extended series (61 SAC, 21 hmMSI-H). An analysis of genes possibly regulated by miR-181a-2* was carried out and, amongst these, an inverse correlation of NAMPT with miR-181a-2* expression was observed, whereas, for TRAF1 and SALL1, additional regulation mechanisms involving CpG island methylation were observed. miR-181a-2* is associated with particular histological and molecular features of colorectal carcinomas within the serrated pathological pathway and might play a role in the immune responses of microsatellite instability carcinomas.
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