Capsid opening enables genome release of iflaviruses.

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Authors

ŠKUBNÍK Karel SUKENÍK Lukáš BUCHTA David FÜZIK Tibor PROCHÁZKOVÁ Michaela MORAVCOVÁ Jana ŠMERDOVÁ Lenka PŘIDAL Antonín VÁCHA Robert PLEVKA Pavel

Year of publication 2021
Type Article in Periodical
Magazine / Source Science advances
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.science.org/doi/pdf/10.1126/sciadv.abd7130
Doi http://dx.doi.org/10.1126/sciadv.abd7130
Keywords DEFORMED WING VIRUSBEE PARALYSIS VIRUSCRYO-EMMOLECULAR-DYNAMICSFORCE-FIELDX-RAYPOLIOVIRUSRNAMODELTRANSITIONS
Description The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.
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