NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses


TIDU Federico DE ZUANI Marco JOSE Shyam Sushama BENDICKOVA Kamila KUBALA Lukáš CARUSO Frank CAVALIERI Francesca FORTE Giancarlo FRIČ Jan

Year of publication 2021
Type Article in Periodical
Magazine / Source iSCIENCE
MU Faculty or unit

Faculty of Medicine

Keywords NFAT signaling; human mesenchymal stromal cells
Description Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being tested as a treatment for graft-versus-host disease (GvHD). The immunosuppressive properties of MSCs seem beneficial; however, their response during fungal infection, which is an important cause of mortality in patients with GvHD, is unknown. We report that MSCs phagocytose the fungal component zymosan, resulting in phosphorylation of spleen tyrosine kinase (Syk), increase in cytosolic calcium levels, and ultimately, increase in NFAT1 nuclear translocation. RNA sequencing analysis of zymosan-treated MSCs showed that CN-NFAT inhibition affects extracellular matrix (ECM) genes but not cytokine expression that is under the control of the NF-kappa B pathway. When coculturing MSCs or decellularized MSC-ECM with human peripheral blood mononuclear cells (PBMCs), selective NFAT inhibition in MSCs decreased cytokine expression by PBMCs. These findings reveal a dual mechanism underlying the MSC response to zymosan: while NF-kappa B directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling.
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