G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability

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Authors

EEKHOUT T. DVOŘÁČKOVÁ Martina GARCIA J.A.P. NEŠPOR DADEJOVÁ Martina KALHORZADEH P. VAN DEN DAELE H. VERCAUTEREN I. FAJKUS Jiří DE VEYLDER L.

Year of publication 2021
Type Article in Periodical
Magazine / Source Plant Physiology
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://academic.oup.com/plphys/article/186/4/1893/6270794
Doi http://dx.doi.org/10.1093/plphys/kiab201
Keywords DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION
Description The WEE1 and ATM AND RAD3-RELATED (ATR) kinases are important regulators of the plant intra-S-phase checkpoint; consequently, WEE1(KO) and ATR(KO) roots are hypersensitive to replication-inhibitory drugs. Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. We demonstrate that lack of FAS1 activity results in the activation of an ATAXIA TELANGIECTASIA MUTATED (ATM)- and SUPPRESSOR OF GAMMA-RESPONSE 1 (SOG1)-mediated G2/M-arrest that renders the ATR and WEE1 checkpoint regulators redundant. This ATM activation accounts for the telomere erosion and loss of ribosomal DNA that are described for fast plants. Knocking out SOG1 in the fast weel background restores replication stress sensitivity, demonstrating that SOG1 is an important secondary checkpoint regulator in plants that fail to activate the intra-S-phase checkpoint.
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