Designované a syntetizované geny kódující nově zkonstruované proteinové katalyzátory (luciferázy) s ověřenými vlastnostmi

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Title in English Designed and synthesized genes coding for new luciferase enzymes with confirmed properties
Authors

MAREK Martin DAMBORSKÝ Jiří BEDNÁŘ David PLUSKAL Daniel MAJEROVÁ Marika PLANAS IGLESIAS Joan

Year of publication 2021
MU Faculty or unit

Faculty of Science

Description Three new coelenterazine-powered luciferase variants were computationally designed. The corresponding genes were synthesized and recombinant proteins were expressed in Escherichia coli. The proteins were purified to high homogeneity. All newly designed proteins showed high expressibility and solubility. Luciferase activities were then determined, revealing that the new enzymes are better in catalysis and light emission than those previously published.
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