PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE
| Authors | |
|---|---|
| Year of publication | 2021 |
| Type | Article in Periodical |
| Magazine / Source | ACTA POLONIAE PHARMACEUTICA |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.ptfarm.pl/download/?file=File%2FActa_Poloniae%2F2021%2F6%2F801.pdf |
| Doi | http://dx.doi.org/10.32383/appdr/146287 |
| Keywords | GnRH analogsmicroparticlessolvent evaporationgelatinesustained drug releasefish reproduction |
| Description | Poly(lactide-co-glycolide) PLGA microparticles represent an efficient and modern tool to encapsulate peptide drugs, which enables their administration to live organisms with the benefit of prolongedrelease. One area that could make the best of this opportunity is fish breeding in aquaculture. The presented study was centered on the formulation of gonadotropin-releasing hormone (GnRH) analog loaded PLGA microparticles intended for fish breeding augmentation, using the double emulsion evaporation method. In this initial experiment, the influence of several input variables (drug, PLGA type, emulsifier concentration, gelatine concentration in internal phase) on observed parameters (morphology, particle size, drug content, drug release, resuspension index) was evaluated. It was found that at lower emulsifier concentration, the particle size is apparently lower (8.54 +/- 6.13-10.36 +/- 4.65 vs. 25.56 +/- 18.86), which is more advantageous in injection administration. Encapsulation efficiency ranged from 39.13 +/- 8.85 to 75.30 +/- 8.83, favoring lower emulsifier concentration, while resuspension index (70.99 +/- 6.47%) suggested the possibility of longer-term administration. Dissolution tests revealed prolonged release for ten days, with most of the drug released in 72-96 hrs. A follow-up study discerning polymer type/gelatine concentration was suggested. Accompanying studies of imaging agent coumarin-6 encapsulation for eventual distribution imaging and metoclopramide base drug release for potential adjuvant use were also successfully realized. |
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