Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to the phage phi 29 DNA


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Year of publication 1991
Type Article in Periodical
Magazine / Source Folia Microbiologica
MU Faculty or unit

Faculty of Science

Field Microbiology, virology
Keywords phi29
Description Partially purified DNA-dependent RNA polymerase of Streptomyces granaticolor was further separated on phosphocellulose in 50% glycerol and a single activity peak was obtained. The enzyme isolated in this way consisted of 4 main proteins with molar mass of 145, 132, 50 and 46kg/mol. These four subunits represented 93% proteins of the active fraction. To test the ability of RNA polymerase to recognize specific sites on DNA, binding sites for RNA polymerase on phage phi 29 DNA were mapped by electron microscopy. The specific binding sites were compared with those for RNA polymerases from Escherichia coli and Bacillus subtilis.

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