Charakterizace nového stafylokokového podoviru a jeho interakce s hostitelem


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Title in English Characterization of a novel staphylococcal podovirus and its interaction with the host


Year of publication 2022
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Detailed characterization of novel bacteriophages is essential for their successful use in the treatment of bacterial infections. However, lytic phages useful for the treatment of infections caused by Staphylococcus sciuri are rarely studied, despite the fact that S. sciuri strains can play a significant role in human medicine and cause infections in livestock with a large economic impact. This study focuses on the unique podovirus vB_SscP-s10 (S10) infecting S. sciuri and its interactions with this bacterial species. The virion of the studied phage consists of an icosahedral head and a short noncontractile tail. Comparison of the S10 proteome with the morphologically similar S. aureus P68 phage showed significant sequence differences, but proteins with analogous function had approximately the same molecular mass. At the genome level, S10 showed the highest similarity to the S. epidermidis Pike phage. The similarity is limited to only 11% of the sequence, which shows 80% identity, indicating the complete uniqueness of this newly described phage. The life cycle of phage S10 lasts 20 min, with a phage yield of 122-163 PFU per infected cell. This is significantly higher than other described staphylococcal podoviruses. The host range of phage S10 is quite narrow. S10 was only able to multiply on two strains of S. sciuri and one strain of S. cohnii out of a total of 80 strains of 19 bacterial species tested. However, the development of resistance to phage S10 was observed even in susceptible strains, and in one of the strains this resistance was confirmed to be due to the presence of phage S10 in a latent form. Approximately 9% of the cells in the bacterial population then underwent lysis associated with spontaneous production of phage S10. This pseudolysogenic state will be further studied to reveal its mechanism.
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