Occurrence of antibiotic-resistant bacterial strains isolated in poultry

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Authors

KOLÁŘ Milan PANTŮČEK Roman BARDOŇ Jan VÁGNEROVÁ Iva TYPOVSKÁ H. VÁLKA Ivo DOŠKAŘ Jiří

Year of publication 2002
Type Article in Periodical
Magazine / Source Veterinary Medicine - Czech
MU Faculty or unit

Faculty of Science

Citation
Web [Full Text]
Field Genetics and molecular biology
Keywords Vancomycin Resistance; Enterococcus; Staphylococcus; Pulsed Field-Gel Electrophoreris; Plasmid Analysis; Poultry
Description The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent both gramnegatives and grampositives, both the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of resistance (in 10% of the strains) were found also against ofloxacin and ciprofloxacin. In staphylococci, increased numbers of strains resistant to erythromycin (39%), clindamycin (19%), tetracycline (14%) and ofloxacin (13%) were observed. In enterococci, 80%, 59% and 34% of the strains were resistant to tetracycline, erythromycin or nitrofurantoin, respectively. A high-level resistance to streptomycin was proved in 22% of the strains. Eleven Enterococcus sp. strains were found to be resistant against vancomycin (vancomycin-resistant enterococci - VRE). Being of the clinical importance, the VRE strains were analysed in detail. Six VRE were identified as Enterococcus faecium VanA, three strains belonged among Enterococcus sp. group III VanB. Remaining two strains were classified as Enterococcus faecium VanB and Enterococcus faecalis VanB, respectively. Based on SmaI macrorestriction analysis, vancomycin-resistant Enterococcus faecium strains, regardless of their resistance type, formed a cluster distinct from control group of vancomycin sensitive strains. Furthermore, among the cluster of vancomycin resistant Enterococcus faecium strains, two clonal lines could be distinguished while the sensitive strains were more heterogenous.
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