Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | Memory in Living Systems |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | p53; Myb; proliferation |
| Description | The v-myb oncogene of AMV causes rapid fatal monoblastic leukemia in chickens. In order to explore the ways to suppress transforming capabilities of the v-Myb we ectopically expressed p53 cDNA in AMV v-myb-transformed chicken monoblasts BM2. p53 can induce expression of multiple genes involved in control of cellular proliferation and apoptosis. Usually, the intracellular concentration of p53 protein is maintained at a very low level. However, DNA damage and other stress stimuli stabilize p53 increasing its cellular concentration. We transfected BM2 cells with human p53 cDNA under control inducible promoter and purified clones of stable transfectants. Stability of human p53 protein in this chicken cells was up-regulated by treatment with roscovitine and adriamycine. p53 did not arrest BM2 cell cycle but it increased apoptosis. Interestingly, induction of apoptosis occurred in p53-expressing BM2 cells in the absence of activation of bax, mdm2 and p21WAF/Cip1. |
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