Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | IFMBE Proceedings, EMBEC 05 |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Keywords | voltage sensitive dye; guinea pig myocardium; ECG; coronary flow; HPLC; electron microscopy |
| Description | Voltage-sensitive dyes are used for recording monophasic action potentials (MAPs) in various heart preparations. Aim of this study was to prove that dye loading has no pharmacological or toxic effects to the preparation, e.g. to prove the viability and quality of the heart at the beginning of MAP recording. The hearts of fifteen guinea pigs were perfused according to Langendorf, loaded with the dye (di-4-ANEPPS) and washed out. During the experiment electrogram and mean coronary flow was recorded. High performance liquid chromatography (HPLC) was used to test on possible ischemic changes of the heart during loading and electron microscopy to check the microscopic changes in the dye exposed tissue. We observed direct effect of di-4-ANEPPS on conductive system and working myocardium of guinea pig heart (changes in heart rate and shape of electrogram). The mean coronary flow does not significantly change during the period of loading with the dye, however during washout decreases. Since no increased production of oxygen free radicals was found and no ultrastructural changes observed, we conclude that the dye application causes vasoconstriction in coronary system without functional or morphological consequences and that loading procedure does not damage the heart muscle and its viability. |
| Related projects: |