Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Physiol. Res. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | cells |
| Description | This study employs mouse embryonal carcinoma (EC) cells P19 to investigate the effects of serum on RA-induced neural differentiation occurring in a simplified monolayer culture. The data show that mouse EC cells are able to produce neural cells upon exposure to RA even without culture in three-dimensional embryoid bodies (EBs). However, in contrast to standard EBs-involving protocol(s), neural differentiation in monolayer only takes place when complex signaling from serum factors is avoided. This simple and efficient strategy is proposed to serve as a basis for neurodifferentiation studies in vitro. |
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