p53 arrests growth and induces differentiation of v-Myb-transformed monoblasts

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Authors

NAVRÁTILOVÁ Jarmila HORVÁTH Viktor KOZUBÍK Alois LOJEK Antonín ŠMARDA Jan

Year of publication 2006
Type Article in Proceedings
Conference ISREC conference on cancer research
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords p53; v-Myb; differentiation; cell cycle; apoptosis
Description v-Myb oncoprotein of AMV interferes with differentiation of myeloid cells. Differentiation of v-Myb-transformed monoblasts BM2 can be resumed by phorbol ester TPA or histone deacetylase inhibitor trichostatin A. The aim of this work was to explore the effects of the p53 tumor suppressor on proliferation, viability, cell cycle progression, apoptosis and differentiation of BM2 cells. We found that ectopic p53 expression completely suppresses proliferation of BM2 cells by imposing block in the G2 phase of the cell cycle. In addition, p53 induces differentiation of BM2 monoblasts to monocytes, as confirmed by up-regulation of production of reactive oxygen species, expression of CD11b, non-specific esterase and myeloperoxidase activities, and by changes of the intracellular pH. In contrast, frequency of apoptosis was not affected by overexpressed p53 in BM2 cells.
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