Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation

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Authors

BRYJA Vítězslav ČAJÁNEK Lukáš GRAHN A. SCHULTE G.

Year of publication 2007
Type Article in Periodical
Magazine / Source Acta Physiologica
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords beta-catenin; clathrin-mediated endocytosis; desensitization
Description We investigated the effects of endocytosis inhibition on Frizzled4-green fluorescent protein (FZD4-GFP) localization, dishevelled levels and Wnt-3a signalling to beta -catenin. Hyperosmotic sucrose and K+ depletion increased the membrane localization of FZD4-GFP, and in parallel triggered fast (1–2 h) and almost complete (approx. 95%) degradation of endogenous dishevelled, which further prevented canonical signalling by Wnt-3a to beta-catenin. The data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation.

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