Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation

Warning

This publication doesn't include Faculty of Medicine. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	BRYJA Vítězslav ČAJÁNEK Lukáš GRAHN A. SCHULTE G.
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Acta Physiologica
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	beta-catenin; clathrin-mediated endocytosis; desensitization
Description	We investigated the effects of endocytosis inhibition on Frizzled4-green fluorescent protein (FZD4-GFP) localization, dishevelled levels and Wnt-3a signalling to beta -catenin. Hyperosmotic sucrose and K+ depletion increased the membrane localization of FZD4-GFP, and in parallel triggered fast (1–2 h) and almost complete (approx. 95%) degradation of endogenous dishevelled, which further prevented canonical signalling by Wnt-3a to beta-catenin. The data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation.