Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation
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| Authors |
BRYJA Vítězslav
ČAJÁNEK Lukáš
GRAHN A.
SCHULTE G.
|
| Year of publication |
2007 |
| Type |
Article in Periodical
|
| Magazine / Source |
Acta Physiologica |
| MU Faculty or unit |
Faculty of Science
|
| Citation |
|
| Field |
Genetics and molecular biology |
| Keywords |
beta-catenin; clathrin-mediated endocytosis; desensitization
|
| Description |
We investigated the effects of endocytosis inhibition on Frizzled4-green fluorescent protein (FZD4-GFP) localization, dishevelled levels and Wnt-3a signalling to beta -catenin. Hyperosmotic sucrose and K+ depletion increased the membrane localization of FZD4-GFP, and in parallel triggered fast (1–2 h) and almost complete (approx. 95%) degradation of endogenous dishevelled, which further prevented canonical signalling by Wnt-3a to beta-catenin. The data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation.
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