Spatio-temporal changes of SDF1 and its CXCR4 receptor in the dorsal root ganglia following unilateral sciatic injury as a model of neuropathic pain

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Year of publication 2010
Type Article in Periodical
Magazine / Source Histochemistry and Cell Biology
MU Faculty or unit

Faculty of Medicine

Web Publikace
Field Neurology, neurosurgery, neurosciences
Keywords Chemokine; Satellite glial cells; Chronic constriction injury
Description There is a growing evidence that chemokines and their receptors play a role in inducing and maintaining neuropathic pain. In the present study, unilateral chronic constriction injury (CCI) of rat sciatic nerve was used to investigate changes for stromal derived factor-1 (SDF1) and its CXCR4 receptor in lumbal and cervical dorsal root ganglia from both sides of naive, operated and sham-operated rats. All operated rats displayed mechanical allodynia and thermal hyperalgesia in hind paws ipsilateral to CCI, but forepaws exhibited only temporal changes of sensitivity. Immunofluorescence staining and Western blot analysis confirmed bilateral alterations of SDF1 and CXCR4 proteins in all examined DRG. No attenuation of mechanical allodynia, but reversed thermal hyperalgesia, in ipsi- and contralateral hind paws was found in CCI-operated rats after i.p. administration of CXCR4 antagonist. These results indicate that SDF1/CXCR4 changes are not limited to DRG associated with injured nerve.
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