Bilateral changes of TNF-alpha and IL-10 protein in the lumbar and cervical dorsal root ganglia following a unilateral chronic constriction injury of the sciatic nerve

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Year of publication 2010
Type Article in Periodical
Magazine / Source Journal of neuroinflammation
MU Faculty or unit

Faculty of Medicine

Field Neurology, neurosurgery, neurosciences
Keywords cytokines; tumor necrosis factor alpha; interleukin 10; behavioral tests; ELISA immunoassay; bilateral changes; neuroinflammation
Description There is a growing evidence that cytokines act as crucial signaling molecules for response of peripheral nerves to injury. We studied levels of tumor necrosis factor alpha (TNF-a) and interleukin 10 (IL-10) proteins using ELISA in the ipsi- and contralateral lumbar and cervical dorsal root ganglia (DRG) from naive rats, rats operated for unilateral chronic constriction injury (CCI) of the sciatic nerve, and sham-operated rats. The ipsilateral hind paws of all operated rats displayed decreased withdrawal thresholds for mechanical allodynia and thermal hyperalgesia, while no significant changes were found in the contralateral hind paws and both forepaws. Bilateral elevation of TNF-a and IL-10 protein was induced in both the lumbar and cervical DRG not only in the homonymous DRG but also in the DRG unassociated with the injured nerve. This suggests that bilaterally increased levels of proteins are linked with general neuroinflammatory reaction to injury rather than only to development and maintenance of neuropathic pain.
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