Transactivation by temperature dependent p53 mutants in yeast and human cells

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Authors

SLOVÁČKOVÁ Jana GROCHOVÁ Diana NAVRÁTILOVÁ Jarmila ŠMARDA Jan ŠMARDOVÁ Jana

Year of publication 2010
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description In response to stress signals, p53 controls essential cell functions by regulating expression of its target genes. Full or partial loss of the p53 function in cancer cells usually results from mutations of the p53 gene. Some of them are temperature-dependent, allowing reactivation of the p53 function in certain temperature.We analyzed transactivation of p21-, bax- and mdm2 genes by 23 temperature-dependent p53 mutants in transiently transfected human lung H1299 cells (p53-null) by luciferase reporter assay.We confirmed temperature-dependency and discriminative character of the most p53 mutants and stratified them into four functional groups. Despite the differences of yeast and human cells, they allowed similar transactivation rates to the p53 mutants, thus providing evidence that functional analysis of separated alleles in yeast is valuable tool for assessment of the human p53 status.
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