Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis

• Authors: HRNČÍŘOVÁ Kristýna; LENGEROVÁ Martina; KOCMANOVÁ Iva; RÁČIL Zdeněk; VOLFOVÁ Pavlína; PALOUŠOVÁ Dita; MOULIS Mojmír; WEINBERGEROVÁ Barbora; WINTEROVÁ Jana; TOŠKOVÁ Martina; POSPÍŠILOVÁ Šárka; MAYER Jiří
• Year of publication: 2010
• Type: Article in Periodical
• Magazine / Source: Journal of Clinical Microbiology
• MU Faculty or unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1128/JCM.01109-10
• Field: Oncology and hematology
• Keywords: Mucormycetes; rapid detection; simple detection; real-time PCR
• Description: We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.