Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase
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| Authors |
ŽERAVÍK Jiří
LACINA Karel
JÍLEK Milan
VLČEK Jiří
SKLÁDAL Petr
|
| Year of publication |
2010 |
| Type |
Article in Periodical
|
| Magazine / Source |
Microchimica Acta |
| MU Faculty or unit |
Faculty of Science
|
| Citation |
|
| Field |
Biochemistry |
| Keywords |
Biosensor; Sarcosine oxidase; Carboxylic acid; Wine;
|
| Description |
The flow-through amperometric biosensor is presented for determination of carboxylic acids. It is based on two sensor layers that are deposited on a platinum electrode. The inner layer serves to eliminate interferences by limiting diffusion of electrochemically active substances such as ascorbic acid. This layer is electro-polymerized using an equimolar mixture of o-phenylenediamine and resorcinol. The outer layer is prepared by cross-linking the enzyme sarcosine oxidase and bovine serum albumin using glutaraldehyde. The formation of enzymatically produced hydrogen peroxide is monitored at 600 mV vs. an Ag/AgCl reference electrode. The addition of carboxylic acids causes competitive inhibition of the enzyme and a decrease in signal. The assay was optimized for determination of carboxylic acids in wine samples. Following 10-fold dilution, most samples contain 1-10 mM individual carboxylic acids and thus a 5 mM concentration of sarcosine was chosen as being optimal for competition.
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