Imbalance in T-cell and cytokine profiles in patients with relapsing-remitting multiple sclerosis

• Authors: MIKULKOVÁ Zuzana; PRAKSOVÁ Petra; ŠTOURAČ Pavel; BEDNAŘÍK Josef; MICHÁLEK Jaroslav
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Journal of the neurological sciences
• MU Faculty or unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.jns.2010.08.053
• Field: Neurology, neurosurgery, neurosciences
• Keywords: multiple sclerosis; T-cells; memory cells; cytokines; flow cytometry
• Description: In the present study, T-lymphocyte populations and pro-inflammatory as well as suppressive cytokine profiles were evaluated in detail in previously untreated patients with relapsing-remitting multiple sclerosis (RRMS). CD4(+) and CD8(+) naive, central memory (Tcm), effector memory (Tem), terminal effector memory (Ttem), CD4(+) regulatory T-cells (Treg) and CD8(+) T-suppressor cells (Ts) were analysed using flow cytometry, and levels of ten plasma cytokines were determined using fluorescent bead-based immunoassay. We evaluated two groups of RRMS with minor (n=33) and major (n=25) clinical impairment and compared them with healthy controls (n=40) in order to detect any correlation between severity of MS clinical symptoms and immune disturbances. In conclusion, we demonstrate complex immune dysbalances in untreated MS patients.

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