Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population



Year of publication 2011
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Periodontitis is an inflammatory disease induced by colonization of the dentogingival interface with pathogenic bacteria and characterized by the connective tissue loss and alveolar bone destruction. Interleukin-8 (IL-8), a CXC chemokine with multiple biological functions, is an important inflammatory mediator responsible for the migration and activation of neutrophils. IL-8 is involved in the initiation and amplification of acute inflammatory reactions and chronic inflammatory processes. The aim of this study was to associate the two single nucleotide polymorphisms in IL-8 with periodontitis and subgingival bacterial colonization in the Czech population. In this case-control association study, a total of 489 unrelated Czech subjects were included. Genomic DNA of 270 patients with chronic periodontitis (CP), 64 patients with aggressive periodontitis (AgP) and 155 healthy/non-periodontitis controls was genotyped using Real-time PCR method for IL-8 gene polymorphisms (RS4073 -251 T/A and RS2227307 +396 T/G). Subgingival bacterial colonization (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Peptostreptococcus micros, Fusobacterium nucleatum in subgingival pockets) was investigated by the DNA-microarray based on a periodontal pathogen detection kit in a subgroup of subjects (N=169). Allele and genotype frequencies of both investigated polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (P>0.05). However, F. nucleatum occurred less frequently in non-periodontitis individuals positive for the T allele of IL-8 +396 T/G variant (OR = 0.10, confidence interval (CI): 0.01-0.82, P<0.01). In contrast, IL-8 -251 T and +396 T allele carriers had an increased odds ratio (OR) for the individual presence of A. actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) in AgP patients (OR = 17.0, confidence interval (CI): 1.85-156.27, P<0.01 for -251 T/A and OR = 10.11, confidence interval (CI): 1.05-97.01, P<0.05 for +396 T/G). Although the IL-8 variants could be shown to be associated with subgingival colonization with A. actinomycetemcomitans in AgP, our findings indicate that these polymorphisms alone are not associated with susceptibility to CP or AgP in the Czech population. This study was supported by projects 1M0528 and IGA NT11405-6.
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