DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL)

• Authors: DRANDI Daniela; BEŠŠE Lenka; FERRERO Simone; DANI Nadia; PASSERA Roberto; MANTOAN Barbara; GAMBELLA Manuela; SARACI Elona; MONITILLO Luigia; GENUARDI Elisa; BARBERO Daniela; GHIONE Paola; GUARONA Guglielmo; OMEDE Paola; BARBERIO Davide; HÁJEK Roman; VITOLO Umberto; CORTELAZZO Sergio; PALUMBO Antonio; BOCCADORO Mario; INGHIRAMI Giorgio; LADETTO Marco
• Year of publication: 2014
• Type: Conference abstract
• MU Faculty or unit: Faculty of Medicine
• Description: Minimal residual disease (MRD) detection is a well described prognostic tool for response evaluation in several hematological malignancies. Real time quantitative PCR (RQ-PCR) has an established role in Mantle cell Lymphoma (MCL), Follicular Lymphoma (FL) and Multiple Myeloma (MM). However, RQ-PCR-based MRD detection is a relative quantification approach, since it requires a reference standard curve. Droplet Digital PCR (ddPCR), based on wide sample partition and Poisson’s statistic, allows for a trustworthy absolute quantification.