Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

Authors

ASHRAFI Amir M SELCUK Ozge MUKHERJEE Atripan UNAL Didem Nur KURBANOGLU Sevinc USLU Bengi JUŘICA Jan PEKARKOVA Jana RICHTERA Lukas ADAM Vojtech

Year of publication 2024
Type Article in Periodical
Magazine / Source Biosensors and Bioelectronics
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.sciencedirect.com/science/article/pii/S0956566324005505
Doi http://dx.doi.org/10.1016/j.bios.2024.116545
Keywords Mercury thin film electrode; Adsorptive stripping voltammetry; Uracil determination; Dihydropyrimidine dehydrogenase phenotyping; 5-Fluorouracil-based therapies
Description Determination of plasma uracil was reported as a method for evaluation of Dihydropyrimidine dehydrogenase (DPD) activity that is highly demanded to ensure the safe administration of 5-fluorouracil (5-FU)-based therapies to cancer patients. This work reports the development of a simple electroanalytical method based on adsorptive stripping square wave voltammetry (AdSWV) at mercury film-coated glassy carbon electrode (MF/GCE) for the highly sensitive determination of uracil in biological fluids that can be used for diagnosis of decreased DPD activity. Due to the formation of the HgII–Uracil complex at the electrode surface, the accuracy of the measurement was not affected by the complicated matrices in biological fluids including human serum, plasma, and urine. The high sensitivity of the developed method results in a low limit of detection (?1.3 nM) in human plasma samples, falling below the practical cut-off level of 15 ng mL-1 (?0.14 µM). This threshold concentration is crucial for predicting 5-FU toxicity, as reported in buffer, and ?1.15% in biological samples), and accuracy (recovery percentage close to 100%).

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