Co-culture with CD40L, IL4 and IL21 expressing stromal cells induces robust CLL cell proliferation in vitro and in PDX co-transplantations

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Authors	HOFERKOVÁ Eva FILIP Daniel ONDRIŠOVÁ Laura ŠEDA Václav VERNER Jan MATULOVÁ Květoslava SKUHROVÁ FRANCOVÁ Hana BOUDNÝ Miroslav VEČEŘA Josef KACZ Péter HLAVÁČ Kryštof PAVELKOVÁ Petra KOŠŤÁLOVÁ Lenka MICHAELOU Androniki POSPÍŠILOVÁ Šárka DORAZILOVA Jana DOUBEK Michael VOJTOVA Lucy KŘEN Leoš MAYER Jiří MRÁZ Marek
Year of publication	2024
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Attached files	Co-culture_with_CD40L__IL4_and_IL21_expressing_stromal_cells_induces_robust_CLL_cell_proliferation_in_vitro_and_in_PDX_co-transplantations.pdf
Description	Several in vitro model systems have been developed to mimic CLL proliferation in lymph nodes typically utilizing CD40L-expressing stromal cells and addition of recombinant factors such as IL4 or IL21 or CpG. However, in such models, CLL proliferation is relatively low and variable. Here we aim to prepare a novel co-culture model based on mimicking T cell signals in vitro and in PDX. We prepared six cell lines by engineering by HS5 stromal cells with stable exypression of human CD40L, IL4. IL21, and their combinants, namely HS5-CD40L, HS5-IL4, HS5-IL21, HS5-CD40L-IL4, HS5-CD40LLOW-IL4, and HS5-CD40L-IL4.IL21 cells. HS5 cells were co.cultured with CLL cells for proliferation and gene expression studies, drug testing and co-transplated on 3D scaffolds into NSG mice.
Related projects:	National institute for cancer research