Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection

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Authors	MIKELOVÁ Radka HODEK P. HANUŠTIAK Pavel ADAM Vojtěch KŘÍŽKOVÁ Soňa HAVEL L. STIBOROVÁ Marie HORNA A. BEKLOVÁ Miroslava TRNKOVÁ Libuše KIZEK René
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Acta Chim. Slov.
MU Faculty or unit	Faculty of Science
Citation
Field	Plant production
Keywords	electrochemical detection; liquid chromatography; isoflavones
Description	Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)