November 1, 2016

Dáša Doležalová Bohačiaková Team

Research Interests | Publications | Funding | Team Members | Collaboration | Meetings | Open Positions

PI M.Sc. Dasa Bohaciakova (Dolezalova), Ph.D.

me-2email: bohaciakova@med.muni.cz
phone: +420 54949 6052

Address:
Kamenice 3/building F1
Department of Histology and Embryology
Faculty of Medicine, Masaryk University Brno
Czech Republic

ORC ID 0000-0002-9538-6668
Masaryk University,
ResearchGate,
Google Scholar

Research interests

  • CURRENT OBJECTIVE:

My group is focused on modeling neural differentiation in vitro using human pluripotent stem cells. We want to understand mechanisms of neural induction and differentiation and use this knowledge to model/treat CNS-related diseases. We currently use numerous neuronal models derived from human pluripotent stem cells, including 2D neurons, neural stem cells, and 3D cerebral organoids, and focus our studies on Alzheimer’s disease and Glioblastoma.

  • AREAS OF INTEREST:

Human Pluripotent Stem Cells, Differentiation, Cerebral organoids, Neurons, Neural Stem/Progenitor Cells, Alzheimer’s disease, Glioblastoma, microRNA, Cell Cycle, DNA damage


Publications

Complete list of publications: click here

2021

2020

2019

2018

2017

  • Bagherpoor AJ, Dolezalova D*, Barta T, Kucirek M, Sani SA, Esner M, Bosakova MK, Vinarsky V, Peskova L, Hampl A, Stros M*. Properties of Human Embryonic Stem Cells and their Differentiated Derivatives Depend on Non-histone DNA-Binding HMGB1 and HMGB2 Proteins. Stem Cells Dev. 2017 Mar 1. PubMed PMID: 27863459; *Corresponding authors
  • Varun D, Srinivasan GR, Tsai YH, Kim HJ, Cutts J, Petty F, Merkley R, Stephanopoulos N, Dolezalova D, Marsala M, Brafman DA. A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs). Acta Biomaterialia. 2017 Jan 15. http://dx.doi.org/10.1016/j.actbio.2016.10.037

2015

  • Juhasova J, Juhas S, Hruska-Plochan M, Dolezalova D, Holubova M, Strnadel J, Marsala S, Motlik J, Marsala M. Time course of spinal doublecortin expression in developing rat and porcine spinal cord: implication in in vivo neural precursor grafting studies. Cellular and molecular neurobiology. 2015 Jan 1. doi:10.1007/s10571-014-0145-7

2014

  • Dolezalova D, Hruska-Plochan M, Bjarkam CR, Sørensen JC, Cunningham M, Weingarten D, Ciacci JD, Juhas S, Juhasova J, Motlik J, Hefferan MP, Hazel T, Johe K, Carromeu C, Muotri A, Bui J, Strnadel J, Marsala M. Pig models of neurodegenerative disorders: Utilization in cell replacement-based preclinical safety and efficacy studies. J Comp Neurol. 2014 Aug 15;522(12):2784-801. doi: 10.1002/cne.23575. Review. PubMed PMID: 24610493.

2013

  • Barta T, Dolezalova D, Holubcova Z, Hampl A. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture. Exp Biol Med (Maywood). 2013 Mar;238(3):271-5. doi: 10.1177/1535370213480711. Review. PubMed PMID: 23598972.

2012

  • Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A. MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells. Stem Cells. 2012 Jul;30(7):1362-72. doi: 10.1002/stem.1108. PubMed PMID: 22511267.
  • Mraz M, Dolezalova D, Plevova K, Stano Kozubik K, Mayerova V, Cerna K, Musilova K, Tichy B, Pavlova S, Borsky M, Verner J, Doubek M, Brychtova Y, Trbusek M, Hampl A, Mayer J, Pospisilova S. MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia. Blood. 2012 Mar 1;119(9):2110-3. doi: 10.1182/blood-2011-11-394874. PubMed PMID: 22234685.

2011

  • Holubcová Z, Matula P, Sedláčková M, Vinarský V, Doležalová D, Bárta T, Dvořák P, Hampl A. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011 Jan;29(1):46-56. doi: 10.1002/stem.549. PubMed PMID: 20960514.

2010

  • Bárta T, Vinarský V, Holubcová Z, Dolezalová D, Verner J, Pospísilová S, Dvorák P, Hampl A. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010 Jul;28(7):1143-52. doi: 10.1002/stem.451. PubMed PMID: 20518019.
  • Borghese L, Dolezalova D, Opitz T, Haupt S, Leinhaas A, Steinfarz B, Koch P, Edenhofer F, Hampl A, Brüstle O. Inhibition of notch signaling in human embryonic stem cell-derived neural stem cells delays G1/S phase transition and accelerates neuronal differentiation in vitro and in vivo. Stem Cells. 2010 May;28(5):955-64. doi: 10.1002/stem.408. PubMed PMID: 20235098.

Funding

ONGOING

01/2022-12/2023

  • Title: Career Restart – Next generation sequencing in neural development and disease

Principal Investigator: Katerina Amruz Cerna, Ph.D.
Institution: Masaryk University, Brno, Czech Republic

Funding agency: Grant Agency of Masaryk University (GAMU)
Grant Number: MUNI/R/1321/2021

05/2021-12/2024

  • Title: Pathogen-induced senescence as the initiation trigger for the onset of Alzheimer’s disease

Principal Investigator: Dasa Bohaciakova, Ph.D.
Institution: Masaryk University, Brno, Czech Republic

Funding agency: Ministry of Health, Czech Republic
Grant Number: NU21-08-00373

01/2021-12/2023

  • Title: Uncovering molecular mechanisms of Alzheimer’s disease initiation using brain organoids

Principal Investigator: Dasa Bohaciakova, Ph.D.
Institution: Masaryk University, Brno, Czech Republic

Funding agency: Grant Agency of the Czech Republic (GACR)
Grant Number: GA21-21510S

01/2021-12/2022

  • Title: Career Restart – Stem Cell-Based Models of Neurodegeneration

Principal Investigator: Dasa Bohaciakova, Ph.D.
Institution: Masaryk University, Brno, Czech Republic

Funding agency: Grant Agency of Masaryk University (GAMU)
Grant Number: MUNI/R/1697/2020

07/2020-12/2023

  • Title: Identifying and treating SORL1-associated Alzheimer’s disease

Coordinator (Consortium): Henne Holstege
Partner: Dasa Bohaciakova, Ph.D.
Institutions: Amsterdam UMC, The Netherlands / Aarhus University, Denmark / Max-Planck Institute of Biophysics, Germany / Masaryk University, Brno, Czech Republic

Funding agency: The Netherlands Organization for Healthy, Research and Development / Innovation Fund Denmark / Ministry of Education Youth and Sports, Czech Republic / Federal Ministry of Education and Research, Germany
Grant Number:  8F20009

01/2020-12/2022

  • Title: New approaches to overcome therapeutic resistance of glioblastoma multiforme: Focus on cellular senescence and cerebral organoid model

Principal Investigator: Zdenek Hodny, M.D., Ph.D.
CO-PI: Dasa Bohaciakova, Ph.D.
Institution: Institute of Molecular Genetics / University Hospital Hradec Kralove / Masaryk University, Brno, Czech Republic

Funding agency: Grant Agency of the Czech Republic (GACR)
Grant Number: GA20-15728S

05/2019 – 12/2022

  • Title: Clinically relevant biochemical, immunological and cellular biomarkers of AAlzheimer’s disease and aging

Principal Investigator: Pharm.Dr. Zdenek Spacil, Ph.D.
CO-PI: Dasa Bohaciakova, Ph.D.
Institution: Research Centre for Toxic Compounds in the Environment (RECETOX) / Masaryk University, Brno, Czech Republic

Funding agency: Ministry of Health Research Programme 2015 – 2022
Grant Number: NV19-08-00472

CLOSED

10/2020-09/2021

  • Title: Neuronální excitabilita jako klíč k patofyziologii řady neuropsychiatrických onemocnění: snímání aktivity in vitro modelů lidské nervové tkáně technikou multielektrodových polí

Principal Investigator: doc. MUDr. Marketa Bebarova, Ph.D.
CO-PI: Dasa Bohaciakova, Ph.D.
Funding agency: Grant Agency of Masaryk University (GAMU)
Grant Number: MUNI/11/SUP/07/2020

03/2018 – 2020

  • Title: Transformative stem cell-based model of Alzheimer’s disease and advanced analytics to study the role of membrane lipids in the pathogenesis

Principal Investigator: Zdenek Spacil, Ph.D.
Co-PI: Dasa Bohaciakova, Ph.D.
Funding agency: Grant Agency of Masaryk University (GAMU)
Grant Number: MUNI/G/1131/2017

2018 – 2020

  • Title:  Functional studies of miRNAs and protein-coding genes involved in stem cell specification during neural development

Principal Investigator: Dasa Bohaciakova, Ph.D.
Funding agency: Grant Agency of the Czech Republic (GACR)
Grant Number: 18-25429Y

2018/01 – 2019/06

  • Title: Klinicky-využitelné neurální kmenové buňky a studium řízení jejich buněčného dělení

Principal Investigator: Dasa Bohaciakova, Ph.D.
Funding agency: Fond Neuron na podporu vedy – Neuron Impuls

2017

  • Title: In vitro modelování patologie Alzheimerovy choroby pomocí cerebrálních organoidů

Institutional support by Faculty of Medicne MU for junior researchers (2017, PI)

2015 – 2017

  • Title: The role of microRNA in cell cycle regulation and differentiation of human embryonic stem cell-derived neural stem cells

Principal Investigator: Dasa Dolezalova, Ph.D.
Funding agency: Grant Agency of the Czech Republic (GACR)
Grant Number: GJ15-18316Y

2016

  • Title: The role of microRNAs in the biology of human embryonic stem cells

Institutional support by Faculty of Medicine MU for junior researchers (2016, PI)

2009 – 2010

  • Title: The role of microRNAs and protein-coding genes in human embryonic stem cell response to DNA damage

Principal Investigator: M.Sc. Dasa Dolezalova
Funding agency: Grant Agency of Masaryk University (GAMU)
Grant Number: MUNI/G/0132/2009

2007 – 2009

  • Title: Analysis of Effects of Inhibition of Cyclin-Dependent Kinases on Differentiation of Human Embryonic Stem Cells

Principal Investigator: Bc. Dasa Dolezalova
Funding agency: Grant Agency of Masaryk University (GAMU)
Grant Number: MUNI/31/A0001/2007


Team members

lab_pic

POSTDOCTORAL RESEARCHERS & RESEARCH SCIENTISTS

M.Sc. Katerina Amruz Cerna, Ph.D.

M.Sc. Petr Fojtik

M.Sc. Hana Hribkova

M.Sc. Veronika Pospisilova, Ph.D.

M.Sc. Jan Raska, Ph.D.

M.Sc. Jiri Sedmik, Ph.D.

M.Sc. Tereza Vanova, Ph.D.

Ph.D. STUDENTS

M.Sc. Veronika Fedorova

  • Project topic: Cell Cycle Regulation in Human Embryonic Stem Cells and Their Differentiated Derivatives

M.Sc. Simona Vochyanova

  • Project topic: Modelling Alzheimer’s disease in the dish: studying the role of risk factors in the development of AD pathogenesis using induced pluripotent stem cells

BACHELOR STUDENTS

Viktorie Koksova

Petra Orviska

ALUMNI

Martin Barak, M.D. (2016-2021) – independent research project as part of the P-Pool study program

  • Project topic: In vitro Neural Differentiation and Cerebral Organoids

Lina El Rifaie (2017-2019) – Medical Student working on independent research project

  • Project topic: Role of p53 in Neural Differentiation and Cerebral Organoid Development

Bc. Martin Kral – Bachelor thesis Student (2020)

  • Project topic: Modeling Alzheimer’s disease using human induced pluripotent stem cells (iPSCs)

M.Sc. Miroslava Peterkova – Diploma thesis Student (2017)

  • Project topic: The role of microRNA in cell cycle regulation and differentiation of human embryonic stem cell-derived neural stem cells

M.Sc. Veronika Fedorova – Bachelor thesis Student, Diploma thesis Student (2017; 2019)

  • Project topic: Cell cycle control in human embryonic stem cells: role of cyclin-dependent kinase inhibitors
  • Project topic: The Role of p16/INK4A protein in the biology of human embryonic stem cells

Collaboration

Neural Differentiation:

  • University of California San Diego, CA, USA – Martin Marsala, MD Department of Anesthesiology
  • Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic (IAPG AS CR) in Libechov, Czech Republic

RNA work:

Alzheimer’s disease:

Glioblastoma multiforme:


Meetings & Conferences

2018

2016

  • 2016/12 – We became part of the newly forming Alzheimer’s disease consortium. We will be establishing 3D cerebral organoids for the in vitro AD modeling.

Open Positions

Postdoc position in Bioinformatics in Stem Cell Neurobiology

see the detailed info and do not hesitate to contact us!

https://www.muni.cz/o-univerzite/kariera-na-mu/volna-mista/67921