Zuzana Holubcová Group
Organisation of mammalian oocyte and its transition to an early embryo
The Lab of Zuzana Holubcová investigates the molecular and mechanical principles governing oocyte architecture and its remodelling during fertilisation and early development. Using advanced live-cell imaging, micromanipulation, and molecular tools, the team aims to uncover how cytoskeletal dynamics, organelle positioning, and membrane remodelling contribute to successful embryo formation. As part of this effort, a postdoctoral researcher will develop protocols to study these processes in model systems with paternal inheritance of centrosomes, including rabbits, cows, and pigs. The transformation of a mammalian oocyte into a developmentally competent embryo requires the precise spatial organisation of cellular structures and robust regulation of meiotic progression.
Publications:
Porokh, V., Kyjovská, D., Martonová, M., Klenková, T., Otevřel, P., Kloudová, S., & Holubcová, Z. (2024). Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos. Nature Communications
Harasimov, K., Uraji, J., Mönnich, E. U., Holubcová, Z., Elder, K., Blayney, M., & Schuh, M. (2023). Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes. Nature Cell Biology
Vondrakova, J., Frolikova, M., Ded, L., Cerny, J., Postlerova, P., Palenikova, V., ... & Komrskova, K. (2022). MAIA, Fc receptor–like 3, supersedes JUNO as IZUMO1 receptor during human fertilization. Science Advances