Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

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KAHOUNOVÁ Zuzana SLABÁKOVÁ Eva BINÓ Lucia REMŠÍK Ján FEDR Radek BOUCHAL Jan KURFŰRSTOVÁ Daniela VRTĚL Radek ŠTUDENT Vladimír JUREČKOVÁ Lucie POROKH Volodymyr HAMPL Aleš SOUČEK Karel

Druh Článek v odborném periodiku
Časopis / Zdroj Stem Cell Research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.scr.2018.11.006
Klíčová slova prostate cancer
Popis A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5 (TM) Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.
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