Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer

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Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

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VRANOVÁ Vladimíra MOUKOVÁ Lucie KIŠŠOVÁ Miroslava SLÁMOVÁ Iva KUGLÍK Petr

Rok publikování 2011
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Cervical cancer remains one of the most common malignancies among women worldwide in both incidence and mortality. Several premalignant stages can be distinguished in the development of invasive carcinoma, including cervical intraepithelial neoplasia grades I-III (CIN I–III). The genetic basis of progression of CIN I to CIN II/III and to invasive carcinoma remain poorly understood. Although infection of high-risk human papilloma virus (HPV) is recognized as an essential initiating event in cervical tumorigenesis, this alone is not sufficient for the progression to invasive cancer. Some chromosomal aberrations have been associated with the progression of CIN to carcinoma, especially the amplification of the human telomerase gene hTERC (3q26) and protooncogene MYCC (8q24). Recently, Cervical FISH Probe Kit (Abbott-Vysis) was designed for common identification of HPV infected cells and copy number aberration of the hTERC a MYCC genes via fluorescence in situ hybridization (FISH) in cervical cytology specimens. In this study, cytology specimens of cervical carcinoma or cervical intraepithelial neoplasia from exocervix and endocervical canal of 20 women were analyzed using the new Cervical FISH Probe Kit. We determined number of HPV positive cells, status of HPV (episomal or integrated), copy number changes of chromosome hTERC and MYCC gene in all samples and compare the findings with the cytologic and histologic diagnoses. In some cases, oligonucleotide array based comparative genomic hybridization (aCGH) from fresh tumour was done to confirm the FISH findings and to find some more chromosomal changes associated with cervical cancer.
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